CVm6A: A Visualization and Exploration Database for m6As in Cell Lines

• Published in: Cells (Basel, Switzerland) Vol. 8; no. 2; p. 168
• Main Authors: Han, Yujing, Feng, Jing, Xia, Linjian, Dong, Xin, Zhang, Xinyang, Zhang, Shihan, Miao, Yuqi, Xu, Qidi, Xiao, Shan, Zuo, Zhixiang, Xia, Laixin, He, Chunjiang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 17.02.2019; MDPI
• Subjects: Adenosine - analogs & derivatives; Adenosine - metabolism; Animals; Cancer; Cell Line; Data Descriptor; Databases as Topic; Datasets; Genes; Genomes; Humans; Insects; Internet; m6A; Mice; mRNA; N6-methyladenosine; RNA-protein interactions; User-Computer Interface; visualization; m6A; visualization; N6-methyladenosine; cell line
• ISSN: 2073-4409; 2073-4409
• DOI: 10.3390/cells8020168

N6-methyladenosine (m6A) has been identified in various biological processes and plays important regulatory functions in diverse cells. However, there is still no visualization database for exploring global m6A patterns across cell lines. Here we collected all available MeRIP-Seq and m6A-CLIP-Seq datasets from public databases and identified 340,950 and 179,201 m6A peaks dependent on 23 human and eight mouse cell lines respectively. Those m6A peaks were further classified into mRNA and lncRNA groups. To better understand the potential function of m6A, we then mapped m6A peaks in different subcellular components and gene regions. Among those human m6A modification, 190,050 and 150,900 peaks were identified in cancer and non-cancer cells, respectively. Finally, all results were integrated and imported into a visualized cell-dependent m6A database CVm6A. We believe the specificity of CVm6A could significantly contribute to the research for the function and regulation of cell-dependent m6A modification in disease and development.