Studies in Phylogeny, Development of Rapid IdentificationMethods, Antifungal Susceptibility, and Growth Rates of Clinical Strains of Sporothrix schenckii Complex in Japan

• Published in: Medical Mycology Journal Vol. 57; no. 3; pp. E47 - E57
• Main Authors: Suzuki, Rumi, Yikelamu, Alimu, Tanaka, Reiko, Igawa, Ken, Yokozeki, Hiroo, Yaguchi, Takashi
• Format: Journal Article
• Language: Japanese; English
• Published: Japan The Japanese Society for Medical Mycology 2016
• Subjects: Antifungal Agents - pharmacology; Antifungal Agents - therapeutic use; Calmodulin - genetics; Drug Resistance, Fungal; Humans; Japan; Microbial Sensitivity Tests; Microbiological Techniques - methods; Mycology - methods; Phylogeny; Polymerase Chain Reaction - methods; Sequence Analysis; Sporothrix; Sporothrix - drug effects; Sporothrix - genetics; Sporothrix - growth & development; Sporothrix - isolation & purification; Sporothrix globosa; Sporothrix schenckii; Sporothrix schenckii sensu stricto; sporotrichosis; Sporotrichosis - drug therapy; Sporotrichosis - microbiology
• ISSN: 2185-6486; 2186-165X; 1882-0476
• DOI: 10.3314/mmj.16-00005

Sporotrichosis is a fungal infection caused by the Sporothrix species, which have distinct virulence profiles and geographic distributions. We performed a phylogenetic study in strains morphologically identified as Sporothrix schenckii from clinical specimens in Japan, which were preserved at the Medical Mycology Research Center, Chiba University. In addition, we examined the in vitro antifungal susceptibility and growth rate to evaluate their physiological features. Three hundred strains were examined using sequence analysis of the partial calmodulin gene, or polymerase chain reaction（PCR）method using newly designed species-specific primers; 291 strains were Sporothrix globosa and 9 strains were S. schenckii sensu stricto (in narrow sense, s. s.). S. globosa strains were further clustered into two subclades, and S. schenckii s. s. strains were divided into three subclades. In 38 strains of S. globosa for which antifungal profiles were determined, 4 strains (11%) showed high minimal inhibitory concentration (MIC) value for itraconazole. All tested strains of S. schenckii s. s. and S. globosa showed low sensitivity for amphotericin B. These antifungals are used for treatment of sporotrichosis when infection is severe. S. schenckii s. s. grew better than S. globosa; wherein S. globosa showed restricted growth at 35℃ and did not grow at 37℃. Our molecular data showed that S. globosa is the main causal agent of sporotrichosis in Japan. It is important to determine the antifungal profiles of each case, in addition to accurate species-level identification, to strategize the therapy for sporotrichosis.