Measurement of the Caspase-1-Like Activity of Vacuolar Processing Enzyme in Plants

Caspase-like activities are essential to regulate programed cell death in plants. Although no caspase orthologous enzymes with aspartic acid specificity have been identified in plants, vacuolar processing enzyme (VPE) exhibits a caspase-1-like activity. In this chapter, we introduce two methods for...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1743; p. 163
Main Authors Hatsugai, Noriyuki, Hara-Nishimura, Ikuko
Format Journal Article
LanguageEnglish
Published United States 2018
Subjects
Apoptosis
Caspase 1 - metabolism
Caspase Inhibitors - pharmacology
Cysteine Endopeptidases - metabolism
Peptides - metabolism
Plant Proteins - metabolism
Plants - drug effects
Plants - enzymology
Plants - metabolism
VPE
Caspase-1-like activity
Plants
Vacuolar processing enzyme
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Summary:Caspase-like activities are essential to regulate programed cell death in plants. Although no caspase orthologous enzymes with aspartic acid specificity have been identified in plants, vacuolar processing enzyme (VPE) exhibits a caspase-1-like activity. In this chapter, we introduce two methods for the measurement of the caspase-1-like/VPE activity. These methods are based on the cleavage of caspase-1 specific synthetic substrates and on monitoring the active forms of VPE using a biotinylated-inhibitor blot analysis. Both methods are also adaptable to other plant caspase-like activities.
ISSN:1940-6029
DOI:10.1007/978-1-4939-7668-3_15