Enzyme-Free Isolation of Adipose-Derived Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are a population of multipotent cells that can be isolated from various adult and fetal tissues, including adipose tissue. These cells contain enormous clinical and basic research appeal due to their plasticity to differentiate into cells of all germ layers in vitro, cr...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1842; p. 203
Main Authors Sherman, Lauren S, Condé-Green, Alexandra, Kotamarti, Vasanth S, Lee, Edward S, Rameshwar, Pranela
Format Journal Article
LanguageEnglish
Published United States 2018
Subjects
Adipose Tissue - cytology
Biomarkers
Cell Separation - methods
Humans
Immunohistochemistry
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - metabolism
Mesenchymal stem cell
Adipose-derived stem cells
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Summary:Mesenchymal stem cells (MSCs) are a population of multipotent cells that can be isolated from various adult and fetal tissues, including adipose tissue. These cells contain enormous clinical and basic research appeal due to their plasticity to differentiate into cells of all germ layers in vitro, cross allogeneic barriers in vivo, and suppress inflammation. Methods to isolate adipose-derived MSCs (ADSCs) primarily rely on enzymatic digestion of the adipose tissue using harsh enzymes such as collagenase. However, these harsh enzymes are expensive and can have detrimental effects on the ADSCs, including risks of using xenograft components in clinical application. This chapter focuses on methods of isolating ADSCs from adipose tissue without enzymatic digestion.
ISSN:1940-6029
DOI:10.1007/978-1-4939-8697-2_14