Compartmentalization Device of Microenvironment for Analyzing iPSCs Differentiation

ABSTRACT This paper reports on a microdevice for compartmentalizing the solution microenvironment that can fractionate the space surrounding spheroids during the culture and differentiation processes, with the aim of verifying the effects of cell–cell interactions in the solution microenvironment on...

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Published inElectrical engineering in Japan Vol. 218; no. 1
Main Authors Fukai, Daiki, Toyoda, Taro, Takao, Hidekuni, Shimokawa, Fusao, Terao, Kyohei
Format Journal Article
LanguageEnglish
Published Hoboken Wiley Subscription Services, Inc 01.03.2025
Subjects
3D printing
cell differentiation
compartmentalization
Differentiation (biology)
human induced pluripotent stem cells
Spheroids
Three dimensional printing
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Abstract ABSTRACT This paper reports on a microdevice for compartmentalizing the solution microenvironment that can fractionate the space surrounding spheroids during the culture and differentiation processes, with the aim of verifying the effects of cell–cell interactions in the solution microenvironment on iPS cell differentiation. In this paper, we demonstrated that spheroids of iPS cells were successfully formed on the device and fractionated their microenvironment by a detachable compartmentalization frame (DCF) formed by 3D printing technology.
AbstractList This paper reports on a microdevice for compartmentalizing the solution microenvironment that can fractionate the space surrounding spheroids during the culture and differentiation processes, with the aim of verifying the effects of cell–cell interactions in the solution microenvironment on iPS cell differentiation. In this paper, we demonstrated that spheroids of iPS cells were successfully formed on the device and fractionated their microenvironment by a detachable compartmentalization frame (DCF) formed by 3D printing technology.
ABSTRACT This paper reports on a microdevice for compartmentalizing the solution microenvironment that can fractionate the space surrounding spheroids during the culture and differentiation processes, with the aim of verifying the effects of cell–cell interactions in the solution microenvironment on iPS cell differentiation. In this paper, we demonstrated that spheroids of iPS cells were successfully formed on the device and fractionated their microenvironment by a detachable compartmentalization frame (DCF) formed by 3D printing technology.
Author Terao, Kyohei
Shimokawa, Fusao
Toyoda, Taro
Takao, Hidekuni
Fukai, Daiki
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Cites_doi 10.1016/j.jbiosc.2015.04.018
10.1016/j.jbiotec.2010.01.012
10.1038/nbt.1554
10.5650/jos1996.49.1071
10.1088/1758-5090/abb529
10.1016/j.cell.2006.07.024
10.1038/s41598-018-30936-2
10.1182/blood.2022017296
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IEEJ Transactions on Sensors and Micromachines
Translated from Volume 144 Number 7, pages 164–169, DOI
This research was funded by a Grant‐in‐Aid for Scientific Research (23K17737) and a grant under the JST FOREST Program (JPMJFR212D).
10.1541/ieejsmas.144.164
(Denki Gakkai Ronbunshi E).
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References_xml – start-page: 229
  year: 2020
  end-page: 232
– volume: 148
  start-page: 3
  issue: 1
  year: 2010
  end-page: 15
  article-title: Multicellular Tumor Spheroids: An Underestimated Tool Is Catching up Again
  publication-title: Journal of Biotechnology
– volume: 12
  issue: 4
  year: 2020
  article-title: Membrane‐Bottomed Microwell Array Added to Transwell Insert to Facilitate Non‐Contact Co‐Culture of Spermatogonial Stem Cell and STO Feeder Cell
  publication-title: Biofabrication
– volume: 18
  start-page: 592
  issue: 8
  year: 2023
  end-page: 598
  article-title: History and Current Status of Clinical Studies Using Human Pluripotent Stem Cells
  publication-title: Stem Cell Reports
– year: 2004
– volume: 121
  start-page: 105
  issue: 1
  year: 2016
  end-page: 110
  article-title: Effect of Separation Distance on the Growth and Differentiation of Mouse Embryoid Bodies in Micropatterned Cultures
  publication-title: Journal of Bioscience and Bioengineering
– volume: 140
  start-page: 2398
  issue: 22
  year: 2020
  end-page: 2402
  article-title: iPLAT1: The First‐in‐Human Clinical Trial of iPSC‐Derived Platelets as a Phase 1 Autologous Transfusion Study
  publication-title: Blood
– volume: 21
  start-page: 3015
  year: 2020
  end-page: 3024
  article-title: Deconstructing the Third Dimension: How 3D Culture Microenvironments Alter Cellular Cues
  publication-title: International Journal of Molecular Sciences
– volume: 241
  start-page: 12
  issue: 3
  year: 2016
  end-page: 16
  article-title: Importance of the Structuration of Cells in the Differentiation Process of human Induced Pluripotent Stem (hiPS) Cells: Effect of the Size of Embryoid Body on Cardiomyocyte Differentiation of hiPS Cells
  publication-title: Chemical Times
– volume: 2020
  year: 2020
  article-title: Size‐Optimized Microspace Culture Facilitates Differentiation of Mouse Induced Pluripotent Stem Cells Into Osteoid‐Rich Bone Constructs
  publication-title: Steam Cells International
– volume: 27
  start-page: 743
  issue: 8
  year: 2009
  end-page: 745
  article-title: Variation in the Safety of Induced Pluripotent Stem Cell Lines
  publication-title: Nature Biotechnology
– volume: 8
  year: 2018
  article-title: Selective Elimination of Human Induced Pluripotent Stem Cells Using Medium With High Concentration of L‐Alanine
  publication-title: Scientific Reports
– volume: 49
  start-page: 1071
  issue: 10
  year: 2000
  end-page: 1080
  article-title: Pluronic Surfactants
  publication-title: Journal of Japan Oil Chemists' Society
– volume: 126
  start-page: 663
  issue: 4
  year: 2006
  end-page: 676
  article-title: Induction of Pluripotent Stem Cells From Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors
  publication-title: Cell
– ident: e_1_2_7_10_1
  doi: 10.1016/j.jbiosc.2015.04.018
– volume: 241
  start-page: 12
  issue: 3
  year: 2016
  ident: e_1_2_7_15_1
  article-title: Importance of the Structuration of Cells in the Differentiation Process of human Induced Pluripotent Stem (hiPS) Cells: Effect of the Size of Embryoid Body on Cardiomyocyte Differentiation of hiPS Cells
  publication-title: Chemical Times
– ident: e_1_2_7_9_1
  doi: 10.1016/j.jbiotec.2010.01.012
– volume: 21
  start-page: 3015
  year: 2020
  ident: e_1_2_7_7_1
  article-title: Deconstructing the Third Dimension: How 3D Culture Microenvironments Alter Cellular Cues
  publication-title: International Journal of Molecular Sciences
– start-page: 229
  volume-title: Theoretical Biology of the Cell
  year: 2020
  ident: e_1_2_7_8_1
– volume: 2020
  year: 2020
  ident: e_1_2_7_11_1
  article-title: Size‐Optimized Microspace Culture Facilitates Differentiation of Mouse Induced Pluripotent Stem Cells Into Osteoid‐Rich Bone Constructs
  publication-title: Steam Cells International
– ident: e_1_2_7_5_1
  doi: 10.1038/nbt.1554
– ident: e_1_2_7_14_1
  doi: 10.5650/jos1996.49.1071
– ident: e_1_2_7_13_1
  doi: 10.1088/1758-5090/abb529
– volume: 18
  start-page: 592
  issue: 8
  year: 2023
  ident: e_1_2_7_4_1
  article-title: History and Current Status of Clinical Studies Using Human Pluripotent Stem Cells
  publication-title: Stem Cell Reports
– ident: e_1_2_7_2_1
  doi: 10.1016/j.cell.2006.07.024
– ident: e_1_2_7_12_1
– ident: e_1_2_7_6_1
  doi: 10.1038/s41598-018-30936-2
– ident: e_1_2_7_3_1
  doi: 10.1182/blood.2022017296
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Snippet ABSTRACT This paper reports on a microdevice for compartmentalizing the solution microenvironment that can fractionate the space surrounding spheroids during...
This paper reports on a microdevice for compartmentalizing the solution microenvironment that can fractionate the space surrounding spheroids during the...
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SubjectTerms 3D printing
cell differentiation
compartmentalization
Differentiation (biology)
human induced pluripotent stem cells
Spheroids
Three dimensional printing
Title Compartmentalization Device of Microenvironment for Analyzing iPSCs Differentiation
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