Compartmentalization Device of Microenvironment for Analyzing iPSCs Differentiation

ABSTRACT This paper reports on a microdevice for compartmentalizing the solution microenvironment that can fractionate the space surrounding spheroids during the culture and differentiation processes, with the aim of verifying the effects of cell–cell interactions in the solution microenvironment on...

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Published inElectrical engineering in Japan Vol. 218; no. 1
Main Authors Fukai, Daiki, Toyoda, Taro, Takao, Hidekuni, Shimokawa, Fusao, Terao, Kyohei
Format Journal Article
LanguageEnglish
Published Hoboken Wiley Subscription Services, Inc 01.03.2025
Subjects
3D printing
cell differentiation
compartmentalization
Differentiation (biology)
human induced pluripotent stem cells
Spheroids
Three dimensional printing
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Summary:ABSTRACT This paper reports on a microdevice for compartmentalizing the solution microenvironment that can fractionate the space surrounding spheroids during the culture and differentiation processes, with the aim of verifying the effects of cell–cell interactions in the solution microenvironment on iPS cell differentiation. In this paper, we demonstrated that spheroids of iPS cells were successfully formed on the device and fractionated their microenvironment by a detachable compartmentalization frame (DCF) formed by 3D printing technology.
Bibliography:Funding
of
IEEJ Transactions on Sensors and Micromachines
Translated from Volume 144 Number 7, pages 164–169, DOI
This research was funded by a Grant‐in‐Aid for Scientific Research (23K17737) and a grant under the JST FOREST Program (JPMJFR212D).
10.1541/ieejsmas.144.164
(Denki Gakkai Ronbunshi E).
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 14
ISSN:0424-7760
1520-6416
DOI:10.1002/eej.23503