Effect of dietary β-1,3-glucan on resistance to white spot syndrome virus (WSSV) in postlarval and juvenile Penaeus monodon

• Published in: Diseases of aquatic organisms Vol. 36; no. 3; pp. 163 - 168
• Main Authors: CHANG, C.-F, SU, M.-S, CHEN, H.-Y, LO, C.-F, KOU, G.-H, LIAO, I.-C
• Format: Journal Article
• Language: English
• Published: Oldendorf Inter-Research 31.05.1999
• Subjects: Animal aquaculture; Animal productions; Biological and medical sciences; Crustacea; Fundamental and applied biological sciences. Psychology; Invertebrate aquaculture; Marine; Penaeus monodon; Schizophyllum commune; White spot syndrome virus; Host; Supplemented diet; Penaeus monodon; Host virus relation; Young animal; Feeding; Macrura; Crustacea; Infection; Marine environment; Glucan; Beta stereoisomer; Sensitivity resistance; Arthropoda; Viral disease; Developmental stage; Decapoda; Invertebrata; Aquaculture
• ISSN: 0177-5103; 1616-1580
• DOI: 10.3354/dao036163

This study evaluated the effectiveness of dietary incorporation of beta -1,3-glucan from Schizophyllum commune in enhancing the resistance of postlarval (PL15) or juvenile (5.5 plus or minus 0.5 g) Penaeus monodon to white spot syndrome virus (WSSV). The shrimp were fed experimental diets with (2 g kg super(-1) diet) or without beta -1,3-glucan for 15 d (postlarvae) or 10 or 20 d (juveniles). Following these dietary treatments, the postlarvae were challenged by immersion and the juveniles by injection using WSSV solution. After challenge with WSSV, initial mortality was significantly (p < 0.005) lower in all the glucan-fed groups than in the respective non-glucan control groups. In the juvenile groups, glucan supplementation for 20 d rather than 10 d significantly (p < 0.005) enhanced survival. After the WSSV challenge, none of the control diet postlarvae or juveniles survived beyond 4 d and none of the 10 d glucan-fed juveniles survived beyond 6 d. By contrast, some of the glucan-treated postlarvae (12.2%) and some of the 20 d glucan-treated juveniles (20%) were still alive at Day 6. These surviving individuals were reared onwards up to 120 d post-challenge, at which time overall survivals were 5.5% (postlarvae) and 13.3% (juveniles) and body weights were 25 to 30 g (postlarvae) and 30 to 40 g (juveniles). Half of the surviving postlarvae and 58% of the surviving juveniles were 2-step WSSV polymerase chain reaction (PCR) negative 6 d after challenge. All the surviving shrimp were 2-step WSSV PCR negative 120 d after challenge.