Morphological and Molecular Identification of Two Strains of Dermatophytes

In this study, we used traditional morphological and molecular identification methods to preliminarily identify two strains of dermatophytes. The two strains were observed under the micro- scope. And then the dermatophytes were cultured on Sabouraud＇s dextrose agar （SDA）. The 18S rRNA regions of the...

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Published inJournal of Huazhong University of Science and Technology. Medical sciences Vol. 33; no. 6; pp. 917 - 922
Main Author 陈力 史国友 王梅梅 赵琳琳 黄园勇 陈霄磊 袁丽杰 熊亚南 司道文 朱丽华
Format Journal Article
LanguageEnglish
Published Heidelberg Huazhong University of Science and Technology 01.12.2013
Subjects
Arthrodermataceae - cytology
Arthrodermataceae - genetics
Arthrodermataceae - isolation & purification
GenBank
Humans
Hyphae - cytology
Medicine
Medicine & Public Health
PCR产物
RNA, Fungal - genetics
RNA, Ribosomal, 18S - genetics
rRNA基因
Skin - microbiology
分子鉴定
形态学鉴定
皮肤
聚合酶链反应
菌株
dermatophyte
gene sequencing
identification
polymerase chain reaction
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ISSN1672-0733
1993-1352
DOI10.1007/s11596-013-1222-8

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Summary:In this study, we used traditional morphological and molecular identification methods to preliminarily identify two strains of dermatophytes. The two strains were observed under the micro- scope. And then the dermatophytes were cultured on Sabouraud＇s dextrose agar （SDA）. The 18S rRNA regions of the two dermatophyte strains were amplified by polymerase chain reaction （PCR）, and the PCR products were sequenced and compared with GenBank data. BLAST tools and DNAMAN soft- ware were used to analyze the sequences. To further determine highly homologous sequences, a phy- logenetic tree was constructed using the Neighbor-Joining method. The two strains of dermatophytes were identified by traditional morphological identification as Epidermophyton floccosurn and Micro- sporum ferrugineum. The 18S rRNA sequence analyses showed high similarities to Cladosporium cladosporioides isolate Cll5LM-UFPR and Ascomycete sp. LB68A1A2. Epidermophyton and Cladosporium belong to dermatophyte, while Microsporum ferrugineum and Ascomycete belong to mi- crosporum. The two novel strains of dermatophytes were therefore identified as Cladosporium cladosporioides isolate C115LM-UFPR （JN650537, Cladosporium ） and Ascomycete sp. LB68A1A2 （AY770409, Ascomycete sp）.
Bibliography:dermatophyte; identification; polymerase chain reaction; gene sequencing
In this study, we used traditional morphological and molecular identification methods to preliminarily identify two strains of dermatophytes. The two strains were observed under the micro- scope. And then the dermatophytes were cultured on Sabouraud＇s dextrose agar （SDA）. The 18S rRNA regions of the two dermatophyte strains were amplified by polymerase chain reaction （PCR）, and the PCR products were sequenced and compared with GenBank data. BLAST tools and DNAMAN soft- ware were used to analyze the sequences. To further determine highly homologous sequences, a phy- logenetic tree was constructed using the Neighbor-Joining method. The two strains of dermatophytes were identified by traditional morphological identification as Epidermophyton floccosurn and Micro- sporum ferrugineum. The 18S rRNA sequence analyses showed high similarities to Cladosporium cladosporioides isolate Cll5LM-UFPR and Ascomycete sp. LB68A1A2. Epidermophyton and Cladosporium belong to dermatophyte, while Microsporum ferrugineum and Ascomycete belong to mi- crosporum. The two novel strains of dermatophytes were therefore identified as Cladosporium cladosporioides isolate C115LM-UFPR （JN650537, Cladosporium ） and Ascomycete sp. LB68A1A2 （AY770409, Ascomycete sp）.
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ISSN:1672-0733
1993-1352
DOI:10.1007/s11596-013-1222-8