Anti-phospholipase A2 and anti-inflammatory activity of Santolina chamaecyparissus

• Published in: Life sciences (1973) Vol. 66; no. 2; pp. PL35 - PL40
• Main Authors: Sala, A, Recio, M C, Giner, R M, Máñez, S, Ríos, J L
• Format: Journal Article
• Language: English
• Published: Netherlands 2000
• Subjects: Animals; Anti-Inflammatory Agents, Non-Steroidal - chemistry; Anti-Inflammatory Agents, Non-Steroidal - isolation & purification; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Asteraceae - chemistry; Enzyme Inhibitors - chemistry; Enzyme Inhibitors - isolation & purification; Enzyme Inhibitors - pharmacology; Female; Flavonoids - chemistry; Flavonoids - isolation & purification; Flavonoids - pharmacology; Mice; Phospholipases A - antagonists & inhibitors; Phospholipases A2; Plant Extracts - pharmacology; Plants, Medicinal - chemistry; Sesquiterpenes - chemistry; Sesquiterpenes - isolation & purification; Sesquiterpenes - pharmacology
• ISSN: 0024-3205
• DOI: 10.1016/s0024-3205(99)00578-0

The activity of the Santolina chamaecyparissus methanol extract was tested against the phospholipase A2 (PLA2)-induced mouse paw edema and in vitro inhibition of PLA2 activity. After fractionation, only the dichloromethane extract was active against the PLA2 in vitro test. In addition, it reduced the edema induced by arachidonic acid, and by 12-O-tetradecanoylphorbol-13-acetate in a multidose test. After chromatography on silicagel and gel filtration on Sephadex, and using an in vitro anti-PLA2 assay-guided process, we have isolated and identified from the dichloromethane extract the flavone nepetin and four sesquiterpenes.