Identification and Structural Characterization of Anti-Endoplasmic Reticulum Stress Compounds from Ageratum conyzoides (L.)

• Published in: Records of natural products Vol. 15; no. 2; pp. 82 - 90
• Main Authors: Binh, Tran Duy, Kusunoki, Ryo, Nguyen, Thanh Q. C., Nakagami, Ako, Kanaori, Kenji, Nguyen, Yen D. H., Pham, Tuan L. A., Nguyen, Cuong C., Vang, Le Van, Kamei, Kaeko
• Format: Journal Article
• Language: English
• Published: Gebze ACG Publications 01.03.2021
• Subjects: Apoptosis; Cell death; Endoplasmic reticulum
• ISSN: 1307-6167; 1307-6167
• DOI: 10.25135/rnp.194.20.06.1697

Ageratum conyzoides is an herbaceous plant widely used in traditional medicine against various diseases, and possesses anti-inflammatory, anti-ulcer, analgesic, and diuretic properties. However, its effects on endoplasmic reticulum (ER) stress, as observed in diseases such as diabetes, have not yet been characterized. In this study, using mouse pancreatic Beta-cells, we demonstrated that the ethanol extract of A. conyzoides effectively suppressed cell death induced by ER stress. Four polymethoxyflavones (PMFs) including (1) 5,6,7,3′,4′,5′- hexamethoxyflavone; (2) 5,6,7,8,3′,4′-hexamethoxyflavone (nobiletin); (3) 3′,4′-methylenedioxy-5′,5,6,7- tetramethoxyflavone; and (4) 5,6,7,8,5′-pentamethoxy-3′,4′-methylenedioxyflavone were isolated from the ethyl acetate fraction using reverse-phase chromatography. Their structures were elucidated using spectroscopic and mass-spectrometric analyses including 1D-, 2D-NMR, and HRESI-MS. All compounds were further evaluated for their ability to inhibit ER stress. Our results suggested that only compound 1 did not show an ability to rescue ER stress-induced cell death. Additionally, compound 2 as a nobiletin and its commercial product alleviated the tunicamycin-dependent chop mRNA expression induction. Notably, extremely low concentrations of both compounds exerted cytoprotective activity against tunicamycin-induced ER stress in pancreatic Beta-cells