Molecular Identification and Analysis on Differential Pathogenicity of Cylindrocarpon Species Associated With Ginseng Root Rust Rot in Northeastern China
Panax ginseng C. A. Meyer is one of the most important medicinal herbs in China. It is known for its high medicinal value and economic value. The ginseng root rust rot (RRR) has always been one of the important diseases troubling the ginseng industry. The yield reduction rate of RRR is ~30%. To unde...
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Published in | Frontiers in plant science Vol. 13; p. 894104 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
28.06.2022
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Subjects | |
Online Access | Get full text |
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Summary: | Panax ginseng
C. A. Meyer is one of the most important medicinal herbs in China. It is known for its high medicinal value and economic value. The ginseng root rust rot (RRR) has always been one of the important diseases troubling the ginseng industry. The yield reduction rate of RRR is ~30%. To understand why the
Cylindrocarpon
species bring about the ginseng RRR in Northeastern China, this study isolates 45 strains from samples collected in Liaoning, Jilin, and Heilongjiang provinces. The rDNA-internal transcribed spacer (ITS) sequence was analyzed to identify the pathogenic species. The morphological characteristics of colonies and conidia of each strain on potato dextrose agar (PDA) medium were observed, and the pathogenicity difference between different pathogenic species was analyzed by pricking method and determining the cell wall degrading enzyme activity. The BLAST alignment analysis shows that the homology of rDNA-ITS between 45 strains and
Cylindrocarpon
is more than 99%, among which 28 are identified as
Cylindrocarpon destructans
, three are identified as
C. destructans
var.
destructans
, and 14 are identified as
Ilyonectria robusta
. The colony diameters of all 45 isolated range from 4.21 ± 0.16a to 7.78 ± 0.25c cm after several days of incubation. Among all the species,
I. robusta
has the fastest growth rate, and
C. destructans
var.
destructans
has the slowest growth rate. Pathogenicity test results show that the pathogenicity of
C. destructans
var.
destructans
is the strongest, followed by
C. destructans
.
I. robusta
has relatively weak pathogenicity. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Plant Pathogen Interactions, a section of the journal Frontiers in Plant Science Reviewed by: Yun-Peng Du, Beijing Academy of Agricultural and Forestry Sciences, China; Annamalai Muthusamy, Manipal Academy of Higher Education, India Edited by: Davinder Singh, The University of Sydney, Australia |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2022.894104 |