Drug reservoir function of human amniotic membrane

• Published in: Journal of ocular pharmacology and therapeutics Vol. 27; no. 4; p. 323
• Main Authors: Resch, Miklós D, Resch, Béla E, Csizmazia, Eszter, Imre, László, Németh, János, Szabó-Révész, Piroska, Csányi, Erzsébet
• Format: Journal Article
• Language: English
• Published: United States 01.08.2011
• Subjects: Amnion - metabolism; Amnion - transplantation; Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - pharmacokinetics; Diffusion Chambers, Culture; Humans; Ofloxacin - administration & dosage; Ofloxacin - pharmacokinetics; Ophthalmic Solutions; Permeability; Spectrophotometry, Ultraviolet; Time Factors
• ISSN: 1557-7732
• DOI: 10.1089/jop.2011.0007

The aim of the study was the quantitative pharmacokinetic evaluation of drug release from pretreated amniotic membrane (AM) in vitro. Cryopreserved AM pieces soaked in 3% ofloxacin ophthalmic solution were mounted in vertical Franz-diffusion cell system equipped with autosampler. In vitro release of ofloxacin was determined by quantitative absorbance measurement carried out with a UV spectrophotometer (wavelength 287 nm). Three groups were created according to the duration of soaking: 60 (Group 1), 120 (Group 2), and 180 (Group 3) minutes. Released amount of ofloxacin pro 1 cm(2) of AM (μg/cm(2)) was calculated in the period of 1 to 450 min. Ofloxacin was detectable in the acceptor phase 1 min after mounting in all groups. Until 120 min, rapid increase of released ofloxacin could be observed. From 120 to 450 min, the amount of released ofloxacin showed a slower increasing pattern. Released ofloxacin in Group 1 was significantly lower than in Group 2 after 90 min (19.4±10.4 μg/cm(2), 51.6±20.7 μg/cm(2), respectively, P=0.044). In Group 3, cumulative drug release was higher than in Group at all timepoints. No significant difference could be demonstrated between Groups 2 and 3 at only 1 min timepoint. Significant ofloxacin reservoir capacity of a single human amniotic layer could be demonstrated in vitro. AM acted as an ofloxacin slow release device for upto 7 h in vitro, depending on the duration of pretreatment of AM. Individual pretreatment of AM could increase beneficial effects of AM transplantation, especially in infectious keratitis.