An Evaluation of Sporicidal Treatments against Blown Pack Spoilage Associated Clostridium estertheticum and Clostridium gasigenes Spores

• Published in: Applied sciences Vol. 12; no. 3; p. 1663
• Main Authors: Esteves, Eden, Koolman, Leonard, Whyte, Paul, Gupta, Tanushree B., Bolton, Declan
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.02.2022
• Subjects: Abattoirs; Alkylamines; blown pack spoilage; Bovine serum albumin; Carbon dioxide; Caustic soda; Chlorine; Clostridium; Clostridium estertheticum; Clostridium gasigenes; Disinfectants; Disinfection & disinfectants; Distilled water; Food contamination; Meat; Organic matter; Peracetic acid; Potassium; Serum albumin; Sodium; Sodium hydroxide; Sodium hypochlorite; Spoilage; Spores; sporicidal agents; Terbium; Ireland
• ISSN: 2076-3417; 2076-3417
• DOI: 10.3390/app12031663

Blown pack spoilage (BPS) occurs when meat is cross-contaminated with Clostridium estertheticum or Clostridium gasigenes spores, often from the meat processing environment. This study tested the efficacy of four sporicidal disinfectants commonly used in beef processing plants against C. estertheticum and C. gasigenes spores in a suspension test. D-values were obtained under model ‘clean’ (sterile distilled water, SDW) and ‘dirty’ (3 g/L bovine serum albumin, BSA) conditions. Mean concentration (log10 CFU/mL) were calculated from direct counts. The levels of dipicolinic acid (DPA), indicating damage to the core of these spores, was also monitored using a terbium (Tb)-DPA assay for treatment 1 (peracetic acid as the active ingredient) in SDW and BSA. In SDW sporicidal treatment 3 (containing peroxymonosulphate) was the most effective against C. estertheticum spores but under ‘dirty’ (BSA) conditions sporicidal treatments 1 and 2 were more effective. A similar pattern was obtained with C. gasigenes with treatment 3 being the most effective in SDW but treatment 2 (sodium hypochlorite as the active ingredient) being more effective in BSA. The lower DPA concentrations obtained in SDW versus BSA demonstrated the protective effect of organic matter. It was concluded that meat processors should use a 5% formulation containing sodium hypochlorite, sodium hydroxide and alkylamine oxide to eliminate BPS Clostridial spores in the abattoir.