DNA extraction and pattern of crab and macrobentos from North Sumatran mangrove forest, Indonesia

• Published in: IOP conference series. Earth and environmental science Vol. 912; no. 1; pp. 12046 - 12049
• Main Authors: Harahap, A M, Basyuni, M, Susetya, I E
• Format: Journal Article
• Language: English
• Published: Bristol IOP Publishing 01.11.2021
• Subjects: Amplification; Animal species; Coastal zone; Deoxyribonucleic acid; DNA; Ecosystems; Forest ecosystems; Genetic analysis; Mangrove swamps; Mangroves; Morphology; Phylogeny; Polymerase chain reaction; Sequence analysis; Species; Taxonomy; Terrestrial ecosystems; Indonesia
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/912/1/012046

Abstract Mangrove forest ecosystem is one of the most productive and unique ecosystems that serves to protect coastal areas from various disturbances, as well as provide habitat for various animal species. The large number of crab species and macrobentos in mangrove ecosystems results in frequent errors in the naming of species that have similarities in morphological features. This problem can be solved through a comprehensive approach by combining morphological analysis with genetic analysi.This study aims to report a DNA extraction and PCR amplification prior was used for the identification of crab and macrozoobentos from mangrove forest, North Sumatra. Primer 16S has a conserved area so it is appropriately used in Polymerase Chain Reaction (PCR) and sequencing analysis to determine taxonomy, phylogeny and diversity between specie. Visualization of PCR amplification results with primer16S from crab samples and macrobentos resulting a low DNA band with a size of 206 bp and a high of 678bp