Transcriptomic Analysis of the CNL Gene Family in the Resistant Rice Cultivar IR28 in Response to Ustilaginoidea virens Infection

• Published in: International journal of molecular sciences Vol. 25; no. 19; p. 10655
• Main Authors: Wang, Zuo-Qian, Wang, Yu-Fu, Xu, Ting, Li, Xin-Yi, Zhang, Shu, Chang, Xiang-Qian, Yang, Xiao-Lin, Meng, Shuai, Lv, Liang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 03.10.2024; MDPI
• Subjects: CNLs; Cultivars; Disease; Disease Resistance - genetics; Gene expression; Gene Expression Profiling - methods; Gene Expression Regulation, Plant; Genetic engineering; Genetic research; Genomes; Health aspects; Host-Pathogen Interactions - genetics; Hypocreales - genetics; Immunity (Disease); Infection; Infections; Multigene Family; NLR immune receptor; Oryza - genetics; Oryza - microbiology; Ovaries; Pathogens; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Proteins - genetics; Plant Proteins - metabolism; Proteins; Rice; Transcriptome - genetics; transcriptome analysis; Ustilaginoidea virens; China; Ustilaginoidea virens; transcriptome analysis; CNLs; NLR immune receptor
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms251910655

Rice false smut, caused by , threatens rice production by reducing yields and contaminating grains with harmful ustiloxins. However, studies on resistance genes are scarce. In this study, the resistance level of IR28 (resistant cultivar) to was validated through artificial inoculation. Notably, a reactivation of resistance genes after transient down-regulation during the first 3 to 5 dpi was observed in IR28 compared to WX98 (susceptible cultivar). Cluster results of a principal component analysis and hierarchical cluster analysis of differentially expressed genes (DEGs) in the transcriptome exhibited longer expression patterns in the early infection phase of IR28, consistent with its sustained resistance response. Results of GO and KEGG enrichment analyses highlighted the suppression of immune pathways when the hyphae first invade stamen filaments at 5 dpi, but sustained up-regulated DEGs were linked to the 'Plant-pathogen interaction' (osa04626) pathway, notably disease-resistant protein RPM1 (K13457, CNLs, coil-coiled NLR). An analysis of CNLs identified 245 proteins containing Rx-CC and NB-ARC domains in the Indica genome. Partial candidate CNLs were shown to exhibit up-regulation at both 1 and 5 dpi in IR28. This study provides insights into CNLs' responses to in IR28, potentially informing resistance mechanisms and genetic breeding targets.