Development of graphene-chitosan composite modified screen-printed carbon electrode and its application for detection of rutin

• Published in: IOP conference series. Earth and environmental science Vol. 299; no. 1; pp. 12005 - 12010
• Main Authors: Wahyuni, W T, Arif, Z, Maryam, N S
• Format: Journal Article
• Language: English
• Published: Bristol IOP Publishing 01.07.2019
• Subjects: Chemical sensors; Chitosan; Electrochemical analysis; Electrochemistry; Electrodes; Flavonoids; Graphene; Linearity; Mechanical properties; Performance evaluation; Quantitation; Rutin
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/299/1/012005

Graphene is a prospective material for sensing devices due to its excellent electronic, thermal, and mechanical properties. In this study, low oxygen graphene was synthesized from graphite and nitronium ion using a microwave-assisted method. Graphene was dispersed into 0.05% chitosan solution and deposited by drop casting method on to screen-printed carbon electrode (SPCE) to form graphene-chitosan composite modified SPCE (SPCE-G-chit). The electrochemical performance of SPCE-G-chit was evaluated using flavonoid 3',4',5,7-tetrahydroxyflavone-3β-D-rutinoside (rutin). SPCE-G-chit provides linearity with R2 of 0.9944 for rutin measurement in the concentration range of 5× 10−7 to 1× 10−5 M. Meanwhile, the linearity of rutin at SPCE was lower (R2 0.9396). Limit of detection (LOD) and limit of quantitation (LOQ) of rutin measurement at SPCE-G-chit was lower (0.23 µM and 0.58 µM, respectively) compare to SPCE (LOD = 0.69 µM and LOQ = 1.75 µM, consecutively). The accuracy of rutin measurement at SPCE-G-chit was 99.45% while the accuracy of rutin measurement at SPCE was 72.18%. The result indicated that SPCE-G-chit provides superior analytical performance than SPCE and potential for electrochemical sensor development.