Typical cutaneous small‐vessel vasculitis induced by combined injection of antiphosphatidylserine/prothrombin complex antibody and anti‐LAMP‐2 antibody in normal rats

We previously reported that IgA vasculitis and cutaneous arteritis could be dependently associated with the presence of the antiphosphatidylserine/prothrombin complex (anti‐PS/PT) antibody and lysosomal‐associated membrane protein‐2 (LAMP‐2). The copy number of LAMP‐2 mRNA in skin tissue samples fro...

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Published inJournal of dermatology Vol. 49; no. 12; pp. 1233 - 1237
Main Authors Kawakami, Tamihiro, Nakade, Issei, Tamura, Yuto, Ito, Fuyu, Nishibata, Yuka, Masuda, Sakiko, Tomaru, Utano, Ishizu, Akihiro
Format Journal Article
LanguageEnglish
Published Tokyo Wiley Subscription Services, Inc 01.12.2022
Subjects
Antibodies
antilysosomal‐associated membrane protein‐2 antibody
antiphosphatidylserine/prothrombin complex antibody
Arteritis
Blood vessels
Cell surface
Copy number
cutaneous arteritis
Endothelial cells
Histones
IgA vasculitis
Immunoglobulin A
Injection
Intravenous administration
Leukocytes (neutrophilic)
Membrane proteins
mRNA
Neutrophils
Prothrombin
Skin
small‐vessel vasculitis
Vasculitis
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Summary:We previously reported that IgA vasculitis and cutaneous arteritis could be dependently associated with the presence of the antiphosphatidylserine/prothrombin complex (anti‐PS/PT) antibody and lysosomal‐associated membrane protein‐2 (LAMP‐2). The copy number of LAMP‐2 mRNA in skin tissue samples from rats with cutaneous vasculitis induced by intravenous administration of anti‐PS/PT antibody after subcutaneous histone injection was significantly higher than in those without cutaneous vasculitis. We found LAMP‐2 protein overexpression in neutrophils and vascular endothelial cells of the affected blood vessels in rats with cutaneous vasculitis induced by intravenous administration of anti‐PS/PT antibody after cutaneous priming by histones. We found typical cutaneous small‐vessel vasculitis in the skin of rats given intravenous injection of both anti‐PS/PT antibody and anti‐LAMP‐2 antibody after cutaneous priming by histones. We suggested that the introduction of skin local histones and anti‐PS/PT antibody in serum could move LAMP‐2 to the cell surface of neutrophils and vascular endothelial cells, and that anti‐LAMP‐2 antibody could bridge these cells through antigen‐specific binding in typical cutaneous small‐vessel vasculitis.
Bibliography:Tamihiro Kawakami and Issei Nakade contributed equally.
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ISSN:0385-2407
1346-8138
DOI:10.1111/1346-8138.16523