Azohydromonas aeria sp. nov., isolated from air

• Published in: The journal of microbiology Vol. 58; no. 7; pp. 543 - 549
• Main Authors: Xue, Han, Piao, Chun-gen, Bian, Dan-ran, Guo, Min-wei, Li, Yong
• Format: Journal Article
• Language: English
• Published: Seoul The Microbiological Society of Korea 01.07.2020; Springer Nature B.V; 한국미생물학회
• Subjects: Air Microbiology; Alcaligenaceae - classification; Alcaligenaceae - genetics; Alcaligenaceae - isolation & purification; Bacterial Typing Techniques; Bacteriocins; Bacteriocins - genetics; Base Composition - genetics; Biomedical and Life Sciences; Catalase; Citric acid; Coenzyme Q8; Colour; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Ethanolamine; Fatty acids; Fatty Acids - genetics; Fatty Acids - metabolism; Gene clusters; Gene sequencing; Genes; Genome, Bacterial - genetics; Genomes; Glycerol; Life Sciences; Lipids; Malic acid; Microbial Systematics and Evolutionary Microbiology; Microbiology; Mountains; New species; pH effects; Phospholipids; Phylogeny; Quinones; Riparian environments; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sodium chloride; Sodium citrate; Spores; Terpenes - metabolism; tRNA; Ubiquinone; 생물학; sp. nov; strain t3-1-3; taxonomy; Azohydromonas aeria sp. nov
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-020-9423-x

A grey pink colored bacterium, strain t3-1-3 T , was isolated from the air at the foot of the Xiangshan Mountain in Beijing, China. The cells are aerobic, Gram-stain-negative, non-spore-forming, motile and coccoid-rod shaped (0.9–1.2 × 1.9–2.1 μm). Strain t3-1-3 T was catalase-positive and oxidase-negative and this strain grew at 4–42°C (optimum 28°C), a pH of 4.0–9.0 (optimum pH 7.0) and under 0–2% (w/v) NaCl (optimum 0–1% NaCl). A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain t3-1-3 T was closely related to Azohydromonas riparia UCM-11 T (97.4% similarity), followed by Azohydromonas australica G1-2 T (96.8%) and Azohydromonas ureilytica UCM-80 T (96.7%). The genome of strain t3-1-3 T contains 6,895 predicted protein-encoding genes, 8 rRNA genes, 62 tRNA genes and one sRNA gene, as well as five potential biosynthetic gene clusters, including clusters of genes coding for non-ribosomal peptide synthetase (NRPS), bacteriocin and arylpolyene and two clusters of genes for terpene. The predominant cellular fatty acids (> 10.0% of the total) in strain t3-1-3 T were summed feature 3 (C 16:1 ω 7 c and/or C 16:1 ω 6 c , 37.8%), summed feature 8 (C 18:1 ω 7 c and/or C 18:1 ω 6 c , 29.7%) and C 16:0 (17.3%). Strain t3-1-3 T contained ubiquinone-8 (Q-8) as the predominant respiratory quinone. The polar lipids of strain t3-1-3 T comprised phosphatidyl ethanolamine (PE), phosphatidyl glycerol (PG), diphosphatidyl glycerol (DPG), an unidentified glycolipid (GL), an unidentified aminophospholipid (APL), two unidentified phospholipid (PL1-2) and five unidentified lipid (L1-5). The DNA G + C content of the type strain is 70.3%. The broader range of growth temperature, assimilation of malic acid and trisodium citrate, presence of C 18:3 ω 6 c and an unidentified glycolipid and absence of C 12:0 2-OH and C 16:0 iso differentiate strain t3-1-3 T from related species. Based on the taxonomic data presented in this study, we suggest that strain t3-1-3 T represents a novel species within the genus Azohydromonas , for which the name Azohydromonas aeria sp. nov. is proposed. The type strain of Azohydromonas aeria is t3-1-3 T (= CFCC 13393 T = LMG 30135 T ).