Expression of ICAM-1 and HLA-DR by human conjunctival epithelial cultured cells and modulation by nedocromil sodium

• Published in: Journal of ocular pharmacology and therapeutics Vol. 14; no. 6; p. 517
• Main Authors: Diebold, Y, Calonge, M, Carretero, V, Fernández, N, Herreras, J M
• Format: Journal Article
• Language: English
• Published: United States 01.12.1998
• Subjects: Anti-Allergic Agents - pharmacology; Anti-Inflammatory Agents - pharmacology; Cells, Cultured; Conjunctiva - cytology; Conjunctiva - drug effects; Conjunctiva - metabolism; Epithelial Cells - drug effects; Epithelial Cells - metabolism; HLA-DR Antigens - metabolism; Humans; Immunoenzyme Techniques; Intercellular Adhesion Molecule-1 - metabolism; Interferon-gamma - pharmacology; Interleukin-1 - pharmacology; Nedocromil - pharmacology; Recombinant Proteins
• ISSN: 1080-7683
• DOI: 10.1089/jop.1998.14.517

It is unclear whether conjunctival epithelial cells participate in the development of immune-mediated events. Using a previously reported in vitro system of human conjunctival epithelium, we determined whether conjunctival epithelial cells express two relevant markers in the antigenic presentation process. Moreover, the potential capability of nedocromil sodium, an antiallergic and antiinflammatory drug, to modulate such expression was investigated. Primary cultures of human conjunctival epithelium and Chang conjunctival cells, incubated with or without 100 U/ml IL-1beta and/or IFNgamma for 1, 3 or 6 h, were simultaneously exposed to 10(-5) M nedocromil sodium. The expression of the intercellular adhesion molecule-1 (ICAM-1) and the human leukocyte antigen-DR (HLA-DR) was determined immunocytochemically. Constitutive expression of ICAM-1 and HLA-DR was observed in primary cultures and Chang cells and was minimally affected by incubation with IL-1beta and/or IFNgamma. The addition of nedocromil sodium resulted in complete abolition of HLA-DR expression and a notable reduction in ICAM-1 expression in primary cultures and Chang cells. These results suggest that epithelial cells from human conjunctiva constitutively express ICAM-1 and HLA-DR in vitro and that such expression is downregulated by nedocromil sodium. This may indicate that conjunctival epithelial cells may be another target for this drug.