Following-up changes in red blood cell deformability and membrane stability in the presence of PTFE graft implanted into the femoral artery in a canine model

• Published in: Korea-Australia rheology journal Vol. 26; no. 2; pp. 209 - 215
• Main Authors: Toth, Csaba, Kiss, Ferenc, Klarik, Zoltan, Gergely, Eszter, Toth, Eniko, Peto, Katalin, Vanyolos, Erzsebet, Miko, Iren, Nemeth, Norbert
• Format: Journal Article
• Language: English
• Published: Heidelberg Korean Society of Rheology, Australian Society of Rheology 01.05.2014; 한국유변학회
• Subjects: Characterization and Evaluation of Materials; Chemistry and Materials Science; Complex Fluids and Microfluidics; Food Science; Materials Science; Mechanical Engineering; Polymer Sciences; Soft and Granular Matter; Themed Articles; 공학일반; membrane stability test; mechanical trauma to red blood cell; synthetic vascular graft; red blood cell deformability; animal model
• ISSN: 1226-119X; 2093-7660
• DOI: 10.1007/s13367-014-0023-3

It is known that a moderate mechanical stress can even improve the red blood cells’ (RBC) micro-rheological characteristics, however, a more significant stress causes deterioration in the deformability. In this study, we aimed to investigate the effect of the presence of artificial graft on the RBC deformability and membrane stability in beagles. In the Control group only anesthesia was induced and in the postoperative (p.o.) period blood samplings were carried out. In the Grafted group under general anesthesia, the left femoral artery was isolated, from which a 3.5 cm segment was resected and a PTFE graft (O.D.: 3 mm) of equal in length was implanted into the gap. On the 1 st , 3 rd , 5 th , 7 th and 14 th p.o. days blood was collected the cephalic veins and RBC deformability was determined ektacytometry (LoRRca MaxSis Osmoscan). Membrane stability test consisted of two deformability measurements before and after the cells were being exposed to mechanical stress (60 or 100 Pa for 300 seconds). Compared to the Control group and the baseline values the red blood cell deformability showed significant deterioration on the 3 rd , 5 th and mainly on the 7 th postoperative day after the graft implantation. The membrane stability of erythrocyte revealed marked inter-group difference on the 3rd, 5th and 7th day: in the Grafted group the deformability decreased and during the membrane stability test smaller difference was observed between the states before and after shearing. We concluded that the presence of a PTFE graft in the femoral artery may cause changes in RBC deformability in the first p.o. week. RBC membrane stability investigation shows a lower elongation index profile for the grafted group and a narrowed alteration in the deformability curves due to mechanical stress.