Silver triangular nanoplates – A multifunctional probe for development of a label-free colorimetric glucose biosensors. Application to glucose in foods and medical samples

• Published in: Materials chemistry and physics Vol. 293; p. 126912
• Main Authors: Tran, Hoang V., Tran, Luyen T., Nguyen, Tinh V., Nguyen, Cuong D.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.01.2023
• Subjects: Colorimetric; Glucose; Glucose oxidase (GOx); Hydrogen peroxide; Label-free biosensors; silver Triangular nanoplates (AgTNPs); Hydrogen peroxide; Glucose; Label-free biosensors; silver Triangular nanoplates (AgTNPs); Glucose oxidase (GOx); Colorimetric
• ISSN: 0254-0584; 1879-3312
• DOI: 10.1016/j.matchemphys.2022.126912

We propose here a simple label-free enzymatic-based colorimetric glucose biosensor using silver triangular nanoplates (AgTNPs) as a multifunctional probe that acts as a signal capturer and an optical transducer. The developed colorimetric glucose biosensor can be applied to glucose monitoring in food or pharmaceutical samples. The detection principle of developed enzymatic-based colorimetric glucose biosensor is based on the reaction of glucose oxidase (GOx) with glucose in a sample to obtain gluconic acid and hydrogen peroxide (H2O2). The generated H2O2 will then be recognised by a standing AgTNP solution with a colour change from blue to purple or colourless. This approach allows for glucose detection in a linear concentration range from 0 to 1 mM at low concentrations and from 1 mM to 2 mM at high concentrations of glucose. The limit of detection (LoD) of the developed biosensor is 0.01 mM with high selectivity. The developed glucose sensor was used to determine glucose in drinking samples and in dextrose–5 wt.% glucose intravenous infusion solution as a pharmaceutical representative sample. [Display omitted] •Silver triangular nanoplate (AgTNPs) were synthesized via a simple route.•The etching of AgTNPs by H2O2 could be applied to develop a colorimetric H2O2 sensor.•A colorimetric glucose biosensor was developed basing on AgTNPs combining with GOx.•The glucose biosensor performed high selectivity and sensitivity with LoD of 10 μM.•AgTNPs-based glucose biosensor can be applied for testing glucose in real samples.