Insertion sequence IS6770 modulates potassium symporter kup transcription in Enterococcus faecalis JH2-2 under low pH conditions

• Published in: International journal of food microbiology Vol. 419; p. 110736
• Main Authors: Acciarri, Giuliana, Taborra, Maria Eugenia, Gizzi, Fernan O., Blancato, Victor S., Magni, Christian
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 16.07.2024
• Subjects: Gene regulation; Genome evolution; Potassium homeostasis; Stress; Transposition; Transposition; Genome evolution; Potassium homeostasis; Gene regulation; Stress
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2024.110736

Enterococcus faecalis is a phylogenetically and industrially relevant microorganism associated with Lactic Acid Bacteria. Some strains of this bacterium are employed as probiotics in commercial applications, while others serve as the principal component in starter cultures for artisanal regional cheese production. However, over the last decade, this species has emerged as an opportunistic multiresistant pathogen, raising concerns about its impact on human health. Recently, we identified multiple potassium transporter systems in E. faecalis, including the Ktr systems (KtrAB and KtrAD), Kup, KimA and Kdp complex (KdpFABC). Nevertheless, the physiological significance of these proteins remains not fully understood. In this study, we observed that the kup gene promoter region in the JH2–2 strain was modified due to the insertion of a complete copy of the IS6770 insertion sequence. Consequently, we investigated the influence of IS6770 on the expression of the kup gene. To achieve this, we conducted a mapping of the promoter region of this gene in the E. faecalis JH2–2 strain, employing fluorescence gene reporters. In addition, a transcriptional analysis of the kup gene was executed in a strain derived from E. faecalis V583 that lacks the IS30-related insertion element, facilitating the identification of the transcriptional start site. Next, the expression of the kup gene was evaluated via RT-qPCR under different pH stressful conditions. A strong upregulation of the kup gene was observed at an initial pH of 5.0 in the strain derived from E. faecalis V583. However, the activation of transcription was not observed in the E. faecalis JH2–2 strain due to the hindrance caused by the presence of IS6770. Besides that, our computational analysis of E. faecalis genomes elucidates a plausible association between transposition and the regulation of the kup gene. Remarkably, the ubiquitous presence of IS6770 throughout the phylogenetic tree implies its ancient existence within E. faecalis. Moreover, the recurrent co-occurrence of IS6770 with the kup gene, observed in 30 % of IS6770-positive strains, alludes to the potential involvement of this genomic arrangement in the adaptive strategies of E. faecalis across diverse niches. •The study sheds light on bacterial adaptation and the role of the IS6770 in evolutionary variation in different environments.•IS6770 insertion specifically disrupts the kup gene promoter region, abrogating its induction under acidic conditions.•kup gene expression in Enterococcus faecalis isolated from various niches is impacted by the IS6770 insertion.•This study emphasizes the significance of IS6770 in modifying the kup gene promoter in E. faecalis.