Novel AKT1 mutations associated with cell-cycle abnormalities in gastric carcinoma

• Published in: Personalized medicine Vol. 15; no. 2; pp. 79 - 86
• Main Authors: Ghatak, Souvik, Lalnunhlimi, Sylvine, Lalrohlui, Freda, Pautu, Jeremy L, Zohmingthanga, John, Kunnumakkara, Ajaikumar B, Kumar, Nachimuthu Senthil
• Format: Journal Article
• Language: English
• Published: England Future Medicine Ltd 01.03.2018
• Subjects: Abnormalities; Adult; AKT1 protein; Alternative Splicing; Amino acids; Apoptosis; Bioinformatics; Cancer; Cell cycle; Cell Cycle - genetics; Deoxyribonucleic acid; DNA; Enhancers; Exons; Female; G1 phase; Gastric cancer; Genes; Genetic Predisposition to Disease - genetics; Humans; India; Kinases; Male; Medical prognosis; Middle Aged; Mutation; Pathogenicity; Pathogens; Phosphatase; Point mutation; Polymorphism, Single Nucleotide - genetics; Proteins; Proto-Oncogene Proteins c-akt - genetics; Proto-Oncogene Proteins c-akt - metabolism; Regulation; Risk Factors; RNA Splicing - genetics; Servers; Silencers; Splicing; Stability analysis; Stomach Neoplasms - genetics; Tumor cells; Tumors; India; AKT1; pleckstrin homology domain; exonic splicing enhancers; gastric cancer; cell cycle
• ISSN: 1741-0541; 1744-828X
• DOI: 10.2217/pme-2017-0053

The aim of this study is to identify the AKT1 gene mutation driven pathogenicity in gastric cancer for Mizo population. 50 diffuse-type gastric tumors were analyzed for AKT1 exon 2 and 14 mutations. Cell-cycle aberration was analyzed in the AKT1-mutated samples and the stability of the protein as well as exonic splicing enhancer motifs were examined. The novel mutations, 15553T >A and 25376C >G might affect the exonic splicing enhancers and silencers. Significant decline was observed in the S-phase population in the tumor cells with 15553T >A and 15579G >C mutations suggesting the arrest of G1 phase. The present study is a novel finding of the possible role of AKT1 mutations which might help to identify gastric cancer patients.