Improving the sensitivity of in vitro skin penetration experiments

The institution of a readily-implemented sample screening and data handling procedure for in vitro skin penetration studies yields substantial improvements in sensitivity for distinguishing between formulations, treatments, penetrants, etc. The procedure involves four steps: 1) prescreen the tissue...

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Bibliographic Details
Published inPharmaceutical research Vol. 11; no. 12; p. 1747
Main Authors Kasting, G B, Filloon, T G, Francis, W R, Meredith, M P
Format Journal Article
LanguageEnglish
Published United States 01.12.1994
Subjects
Humans
Permeability
Skin - metabolism
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Summary:The institution of a readily-implemented sample screening and data handling procedure for in vitro skin penetration studies yields substantial improvements in sensitivity for distinguishing between formulations, treatments, penetrants, etc. The procedure involves four steps: 1) prescreen the tissue samples to determine their intrinsic permeability; 2) apply treatments using a randomized complete block (RCB) design, with blocking by tissue permeability; 3) apply a variance-stabilizing transformation to the penetration data, followed by outlier testing; and 4) analyze the transformed data according to an RCB analysis of variance, using tissue permeability as the blocking variable. For penetration studies in which high sample variability is a concern, the above procedure commonly yields a sensitivity advantage of several-fold versus alternative methods of comparison.
ISSN:0724-8741
DOI:10.1023/A:1018915416930