Structural Analysis and Antioxidant Activity of Extracellular Polysaccharides Extracted from Culinary-Medicinal White Jelly Mushroom Tremella fuciformis (Tremellomycetes) Conidium Cells

• Published in: International journal of medicinal mushrooms Vol. 22; no. 5; p. 489
• Main Authors: Zheng, Qianwang, He, Bao-Lin, Wang, Jiu-Ying, Huang, Shi-Shi, Zou, Yuan, Wei, Tao, Ye, Zhiwei, Guo, Liqiong, Lin, Jun-Fang
• Format: Journal Article
• Language: English
• Published: United States 2020
• Subjects: Antioxidants - chemistry; Antioxidants - isolation & purification; Basidiomycota - chemistry; Complex Mixtures - chemistry; Fermentation; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Spectroscopy, Fourier Transform Infrared; Spores, Fungal - chemistry
• ISSN: 1940-4344
• DOI: 10.1615/IntJMedMushrooms.2020034764

Using Tremella fuciformis conidium cells for submerged fermentation is a cost-effective way to harvest bioactive compounds. In this study, we emphasized the structural and functional analysis of extracellular polysaccharides (EPS) extracted from T. fuciformis conidium cells. An EPS high-yield strain tyc63 was selected and a 6-day optimum fermentation period was determined. Crude EPS was extracted and three high molecular weight (5189, 171.6, and 661 kDa) polysaccharides TFP-1, TFP-2, and TFP-3 were isolated and purified. TFP-1 is mainly composed of glucose, xylose, mannose, and fucose, while both TFP-2 and TFP-3 are mainly composed of rhamnose, arabinose, mannose, galactose, and glucose. FT-IR analysis revealed that TFP-1, TFP-2, and TFP-3 have typical polysaccharide structure. The antioxidant assay revealed that the crude EPS, TFP-1, TFP-2, and TFP-3 presented high free radical scavenging activities but low ferric reducing power, suggesting that the EPS produced by liquid fermentation could be used as a potent radical scavenger.