Sulfate-reducing bacterial communities in the water column of the Gdansk Deep (Baltic Sea)
Biodiversity of sulfate-reducing bacterial communities in the water column of the Gdansk Deep, Baltic Sea, where H 2 S had been detected in near-bottom layers, was analyzed by PCR with primers for the 16S rRNA genes of six major phylogenetic subgroups of sulfate-reducing bacteria (SRB). Using denatu...
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Published in | Microbiology (New York) Vol. 84; no. 2; pp. 268 - 277 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Moscow
Pleiades Publishing
01.03.2015
|
Subjects | |
Online Access | Get full text |
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Summary: | Biodiversity of sulfate-reducing bacterial communities in the water column of the Gdansk Deep, Baltic Sea, where H
2
S had been detected in near-bottom layers, was analyzed by PCR with primers for the 16S rRNA genes of six major phylogenetic subgroups of sulfate-reducing bacteria (SRB). Using denaturing gradient gel electrophoresis followed by sequencing, the nucleotide sequences of reamplified
dsrB
gene fragments from investigated water samples were determined. For the first time the presence of nucleotide sequences of the
dsrB
gene was detected by PCR in the water samples from all hydrochemical layers, including subsurface oxic waters. The presence of the 16S rRNA genes of representatives of
Desulfotomaculum, Desulfococcus-Desulfonema-Desulfosarcina
, and
Desulfovibrio-Desulfomicrobium
SRB subgroups was also revealed throughout the water column of the Gdansk Deep. Analysis of translated amino acid sequences encoded by the
dsrB
gene demonstrated the highest homology with the relevant sequences of uncultured SRB from various marine habitats. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0026-2617 1608-3237 |
DOI: | 10.1134/S002626171502006X |