Impact of High Fat Consumption on Neurological Functions after Traumatic Brain Injury in Rats

• Published in: Journal of neurotrauma Vol. 39; no. 21-22; p. 1547
• Main Authors: Thomson, Shannon, Chan, Yik Lung, Yi, Chenju, Wang, Baoming, Machaalani, Rita, Oliver, Brian G, Gorrie, Catherine A, Chen, Hui
• Format: Journal Article
• Language: English
• Published: United States 01.11.2022
• Subjects: Animals; Biomarkers - metabolism; Brain - metabolism; Brain Injuries, Traumatic - complications; Brain Injuries, Traumatic - metabolism; Female; Inflammation - metabolism; Oxidative Stress; Rats; Rats, Sprague-Dawley; macrophages; sensory function; short-term memory; synapse; MnSOD
• ISSN: 1557-9042
• DOI: 10.1089/neu.2022.0080

Traumatic brain injury (TBI) and obesity are two common conditions in modern society; both can impair neuronal integrity and neurological function. However, it is unclear whether the coexistence of both conditions will worsen outcomes. Therefore, in a rat model, we aimed to investigate whether the coexistence of TBI and a high-fat diet (HFD) has an additive effect, leading to more severe neurological impairments, and whether they are related to changes in brain protein markers of oxidative stress, inflammation, and synaptic plasticity. Sprague-Dawley rats (female, ∼250 g) were divided into HFD (43% fat) and diet (CD) (17% fat) groups for 6 weeks. Within each dietary group, half underwent a TBI by a weight-drop device, and the other half underwent sham surgery. Short-term memory and sensory function were measured at 24 h, 1 week, 3 weeks, and 6 weeks post-TBI. Brain tissues were harvested at 24 h and 6 weeks post-TBI, and markers of oxidative stress, apoptosis, inflammation, and synaptic plasticity were measured via immunostaining and Western blotting. In rats without TBI, HFD increased the pre-synaptic protein synaptophysin. In rats with TBI, HFD resulted in worsened sensory and memory function, an increase in activated macrophages, and a decrease in the endogenous antioxidant manganese superoxide dismutase (MnSOD). Our findings suggest that the additive effect of HFD and TBI worsens short term memory and sensation deficits, and may be driven by enhanced oxidative stress and inflammation.