Identification of Genetic on Blood Serum Protein of Prolific Ewes

• Published in: IOP conference series. Earth and environmental science Vol. 119; no. 1; pp. 12031 - 12038
• Main Authors: Sutiyono, Ondho, Y. S., Setiatin, E. T., Sutopo, Laily, A. N., Prasetyowati, D. E., Noviani, F.
• Format: Journal Article
• Language: English
• Published: Bristol IOP Publishing 01.02.2018
• Subjects: Blood; Blood plasma; Data analysis; Electrophoresis; Gel electrophoresis; Gene frequency; Genotypes; Jugular vein; Polyacrylamide; Proteins; Sampling; Serum proteins; Sheep; Statistical analysis; Twins
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/119/1/012031

The aim of the research was to identify the genetic specification of blood plasma protein in ewes that are prolific. The material of study of local sheep in Bawen and Jambu Sub-district of Semarang Regency is 132 which is determined by purposive sampling that have been give lambing three times. Ewes were divided into three groups that always has a single child (L1), ever had twins (L2) and twins more than two (LM2). Blood sampling was performed using dispossible syringe in jugular vein as much as 5 ml per ewe. Blood plasma was analyzed by Polyacrylamide Gel Electrophoresis-Thin Layer (PAGETLE) method in Biochemistry Laboratory of Veterinary Faculty of Gadjah Mada University. Data analysis is using descriptive statistics and the laws of equilibrium Hardy-Weberg. The research parameters were comparison type of ewes and frequency genetic of protein of blood serum. The results showed that the parent comparisons of L1, L2 and LM2 were 66 (50.00%), 49 (37.12%) and 17 (12.88%), respectively. The frequency genes haven a high propensity to relationship of prolificacy nature parent are Pal2, AlbB, CPF, TFB, PTFS and AmlB on pointes, 67.65, 55.88, 91.17, 70.59, 79.41 and 91.18%. Conclusion the mostly LM2 ewes have genotypes Pal1Pal2, AlbBAlbC, CpFCpF, TfATfB, PtfSPtfS and AmlBAmlB whit frequency are 52.94%, 52.94%, 88.24, 47.06, 64.71 and 88.24% respectively.