Antimicrobial susceptibility and genomic characterization of Vibrio parahaemolyticus isolated from aquatic foods in 15 provinces, China, 2020

• Published in: International journal of food microbiology Vol. 418; p. 110737
• Main Authors: Bai, Yao, Yang, Qiuping, Sun, Yanan, Li, Fengqin, Sun, Jiali, Yang, Shuran, Yang, Dajin, Peng, Zixin, Yang, Baowei, Xu, Jin, Dong, Yinping, Yan, Shaofei, Li, Ning
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 16.06.2024
• Subjects: Anti-Bacterial Agents - pharmacology; Antimicrobial susceptibility; Aquatic foods; China - epidemiology; Drug Resistance, Multiple, Bacterial - genetics; Food Microbiology; Genome, Bacterial; Genomic analysis; Genotype; Humans; Microbial Sensitivity Tests; Phylogeny; Seafood - microbiology; Vibrio parahaemolyticus; Vibrio parahaemolyticus - classification; Vibrio parahaemolyticus - drug effects; Vibrio parahaemolyticus - genetics; Vibrio parahaemolyticus - isolation & purification; Vibrio parahaemolyticus - pathogenicity; Virulence Factors - genetics; China; Vibrio parahaemolyticus; Antimicrobial susceptibility; Genomic analysis; Aquatic foods
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2024.110737

Prevalent in marine, estuarine and coastal environments, Vibrio parahaemolyticus is one of the major foodborne pathogens which can cause acute gastroenteritis through consumption of contaminated food. This study encompassed antimicrobial resistance, molecular characteristics and phylogenetic relationships of 163 V. parahaemolyticus isolated from aquatic foods across 15 provinces in China. The isolates showed high resistance rates against ampicillin (90.80 %, 148/163) and cefazolin (72.39 %, 118/163). Only 5 isolates demonstrated multi-drug resistance (MDR) phenotypes. A total of 37 different antibiotic resistance genes (ARGs) in correlation with seven antimicrobial categories were identified. tet(34) and tet(35) were present in all 163 isolates. Other most prevalent ARGs were those conferring resistance to β-lactams, with prevalence rate around 18.40 % (30/163). The virulence genes tdh and trh were found in 17 (10.43 %) and 9 (5.52 %) isolates, respectively. Totally 121 sequence types (STs) were identified through whole genome analysis, among which 60 were novel. The most prevalent sequence type was ST3 (9.20 %, 15/163), which shared the same genotype profile of trh_, tdh+ and blaCARB-22+. Most of the tdh+V. parahaemolyticus isolates was clustered into a distinctive clade by the phylogenetic analysis. Our study showed that the antimicrobial resistance of V. parahaemolyticus in aquatic foods in China was moderate. However, the emerging of MDR isolates implicate strengthened monitoring is needed for the better treatment of human V. parahaemolyticus infections. High genetic diversity and virulence potential of the isolates analyzed in this study help better understanding and evaluating the risk of V. parahaemolyticus posed to public health. •MDR V. parahaemolyticus isolates appeared in a specific region of China.•One trh+ MDR V. parahaemolyticus was found.•The most prevalent sequence type ST3 all harbored tdh and blaCARB.•The majority of the tdh+ isolates were clustered into a distinctive clade.