Identification and functional characterization of a sesquiterpene synthase gene from Eleutherococcus trifoliatus

In the present study, one sesquiterpene synthase gene in Eleutherococcus trifoliatus was identified and characterized. Full-length cDNA was obtained from stems. It contained an open reading frame of 1671 bp (EtCop) with a predicted molecular mass of 64.5 kDa. The amino acid sequence of EtCop contain...

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Bibliographic Details
Published inHolzforschung Vol. 66; no. 2; pp. 183 - 189
Main Authors Wen, Chi-Hsiang, Tseng, Yen-Hsueh, Chu, Fang-Hua
Format Journal Article
LanguageEnglish
Published Walter de Gruyter 01.02.2012
Subjects
Araliceae
Eleutherococcus trifoliatus
sesquiterpene synthase
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Summary:In the present study, one sesquiterpene synthase gene in Eleutherococcus trifoliatus was identified and characterized. Full-length cDNA was obtained from stems. It contained an open reading frame of 1671 bp (EtCop) with a predicted molecular mass of 64.5 kDa. The amino acid sequence of EtCop contained the common terpene synthase family motifs RR(x)8W, RxR and DDxxD. The recombinant protein from Escherichia coli was incubated with farnesyl diphosphate in order to identify the function of EtCop. The product of EtCop could be identified as an α-copaene by means of gas chromatography-mass spectrometry analysis and comparison with an authentic standard.
Bibliography:Corresponding author. School of Forestry and Resource Conservation, National Taiwan University, Taipei 106, Taiwan Phone: +886-2-33665261 Fax: +886-2-23654520
istex:14146C8FB843B11557DEC1CBB1F6DDC2D016F765
ark:/67375/QT4-WPD8RV36-J
ArticleID:hf.2011.135
hf.2011.135.pdf
ISSN:0018-3830
1437-434X
DOI:10.1515/HF.2011.135