Comparative characterization of biogenic and chemical synthesized hydroxyapatite biomaterials for potential biomedical application

• Published in: Materials chemistry and physics Vol. 228; pp. 344 - 356
• Main Authors: Mondal, Sudip, Hoang, Giang, Manivasagan, Panchanathan, Moorthy, Madhappan Santha, Kim, Hye Hyun, Vy Phan, Thi Tuong, Oh, Junghwan
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.04.2019
• Subjects: Biological study; Bone tissue engineering; Fish bone; Hydroxyapatite; Scaffold; Hydroxyapatite; Bone tissue engineering; Fish bone; Scaffold; Biological study
• ISSN: 0254-0584; 1879-3312
• DOI: 10.1016/j.matchemphys.2019.02.021

This present study illustrates the processing and comprehensive characterization of biogenic hydroxyapatite (FB-HAp) biomaterial from fish bone (Tuna fish) in a facile single step thermal decomposition procedure. Fish bones were calcined at 1000 °C in an air atmosphere for 1 h. The obtained FB-HAp flakes were ball milled for 30 h at a unidirectional milling speed of 300 rpm. A well-established wet chemical process was obtained to synthesize HAp nanoparticles (C-HAp). The synthesized non-stoichiometric (Ca/P = 1.87) FB-HAp phases were identified by X-ray diffraction study and compared with C-HAp. The thermal properties were studied by thermogravimetric and differential thermal analysis. Raman spectroscopy and Fourier transform infrared spectroscopy confirmed the structural and functional groups of FB-HAp and C-HAp. The morphological and elemental analysis was performed by transmission electron microscopy, energy dispersive X-ray spectroscopy and Inductive coupled plasma (ICP) spectroscopy. The synthesized FB-HAp and C-HAp were mixed with 2% starch porogen and compacted with ∼200 MPa unidirectional pressure to obtained small pellet scaffolds. The obtained scaffolds were sintered at 1000 °C in an air atmosphere for 2 h and further used for cell attachment and proliferation study. The FB-HAp biomaterials showed non-toxic behavior with MG-63 osteoblast cell lines similar to C-HAp. Additionally, FB-HAp scaffold showed enhanced cellular attachment and proliferation on its surface. The in-depth chemical, structural, and morphological analysis suggest that fish bone could be a promising sustainable biogenic source of HAp for biomedical applications. •Facile synthesis of biogenic hydroxyapatite from waste tuna bones.•Comparative characterization of fish bone and chemically synthesized hydroxyapatite.•Possible mechanism for enhanced bioactivity of biogenic hydroxyapatite.•Scaffold fabrication and promising application for tissue engineering.