Pigment mutants of the green microalga Chlamydomonas reinhardtii: Morphological properties and photosynthetic performance

• Published in: Russian journal of plant physiology Vol. 63; no. 4; pp. 443 - 450
• Main Authors: Sadvakasova, A. K., Akmukhanova, N. R., Zayadan, B. K., Matorin, D. N., Protopopov, F. F., Alekseev, A. A., Bolatkhan, K.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.07.2016
• Subjects: Biomedical and Life Sciences; Chlamydomonas reinhardtii; Life Sciences; Plant Physiology; Plant Sciences; Research Papers; chlorophyll; JIP test; fluorescence; photosynthesis; delayed fluorescence
• ISSN: 1021-4437; 1608-3407
• DOI: 10.1134/S1021443716040130

Pigment mutants CC-124y-1, СС-124у-2, and СС-124р-2 of the green microalga Chlamydomonas reinhardtii Dangeard were obtained by means of UV-induced mutagenesis. Morphological properties and photosynthetic activity of these mutants were examined. The mutants displayed a lowered content of chlorophyll b and carotenoids. Analysis of fluorescence induction curves revealed the decreased rates of electron transport in photosystem II (PSII), the increase in the fraction of Q B -nonreducing centers, and the enhancement of nonphotochemical fluorescence quenching. The mutations had no direct impact on oxidation of pigment P 700 in PSI and on the decline of delayed fluorescence. The parameters of induction curves of prompt and delayed fluorescence are proposed for use in early diagnostics of UV-induced mutagenesis. The pigment mutants obtained can be applied in biomonitoring studies.