New SNP markers of the honeybee vitellogenin gene (Vg) used for identification of subspecies Apis mellifera mellifera L

• Published in: Russian journal of genetics Vol. 51; no. 2; pp. 163 - 168
• Main Authors: Ilyasov, R. A., Poskryakov, A. V., Nikolenko, A. G.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.02.2015
• Subjects: Animal Genetics; Animal Genetics and Genomics; apiculture; Apis mellifera mellifera; Biomedical and Life Sciences; Biomedicine; DNA barcoding; Eurasia; evolution; gene pool; genes; genetic markers; genotyping; honey bee colonies; honey bees; Human Genetics; immunity; longevity; Microbial Genetics and Genomics; selection methods; single nucleotide polymorphism; social structure; vitellogenin; Eurasia; Ural Region; Apis Mellifera; Selective Breeding; Single Nucleotide Polymorphism Marker; Evolutionary Branch
• ISSN: 1022-7954; 1608-3369
• DOI: 10.1134/S1022795415020088

Preservation of the gene pool of honeybee subspecies Apis mellifera mellifera is of vital importance for successful beekeeping development in the northern regions of Eurasia. An effective method of genotyping honeybee colonies used in modern science is the mapping of sites of single nucleotide polymorphism (SNP). The honeybee vitellogenin gene ( Vg ) encodes a protein that affects reproductive function, behavior, immunity, longevity, and social organization in the honeybee Apis mellifera and is therefore a topical research subject. The results of comparative analysis of honeybee Vg sequences show that there are 26 SNP sites that differentiate M and C evolutionary branches and can be used as markers in selective breeding, DNA-barcoding, and the creation of genetic passports for A. m. mellifera colonies.