Assessment of DNA damage caused by locally produced hydroxyapatite-silica nanocomposite using Comet assay on human lung fibroblast cell line

• Published in: Molecular & cellular toxicology Vol. 8; no. 1; pp. 53 - 60
• Main Authors: Musa, Marahaini, Kannan, Thirumulu Ponnuraj, Masudi, Sam’an Malik, Rahman, Ismail Ab
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean Society of Toxicogenomics and Toxicoproteomics 01.03.2012; 대한독성 유전단백체 학회
• Subjects: Biomedical and Life Sciences; Cell Biology; Life Sciences; Original Paper; Pharmacology/Toxicology; 생물학; Hydroxyapatite-silica nanocomposite; MTT assay; DNA damage; Comet assay; Genotoxicity
• ISSN: 1738-642X; 2092-8467
• DOI: 10.1007/s13273-012-0007-7

The growing interest of nanotechnology in dentistry has sparked various applications of biomaterials in nanoscale to be developed. The aim of this study was to evaluate the genotoxicity effect of locally produced hydroxyapatite-silica nanocomposite (School of Dental Sciences, Universiti Sains Malaysia, Malaysia) using Comet assay on human lung fibroblast cell line, MRC-5. Extraction of this test material was prepared and the concentrations which produced IC 10 , IC 25 and IC 50 in cytotoxicity tests (MTT assay) were recorded. Three specific concentrations, 0.00005 g/mL, 0.0009 g/mL and 0.1 g/mL for IC 10 , 1C 25 and IC 50 respectively were further used to evaluate the genotoxicity effect along with concurrent positive (hydrogen peroxide) and negative (Eagle’s Minimum Essential Medium) controls. There was no significant difference in the tail moments between negative control and treatment groups (0.00005 g/mL). Dose-dependent relationship was observed, where significant difference was noticed in the tail moments between 0.0009 g/mL and 0.1 g/mL groups with that of the negative control. However, since the values were still less than 5, it can be considered as non-genotoxic. The tail moments between different concentrations of hydroxyapatite-silica nanocomposite and positive control differed significantly ( P /0.05). This concludes that the locally produced HAsilica nanocomposite is non-genotoxic by Comet assay under the present test conditions.