Development and Validation of a Quantitative Ultra Performance LC® Hydrophilic Interaction Liquid Chromatography MS/MS Method to Measure Fructose and Sorbitol in Human Plasma

Fructose and sorbitol are utilized as biomarkers for nonalcoholic steatohepatitis. Measurement of fructose and sorbitol levels helps understanding disease progression, drug response and underlying mechanism. Stable isotope-labeled fructose and sorbitol were used as surrogate standards and internal s...

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Published inBioanalysis Vol. 11; no. 5; pp. 407 - 425
Main Authors Li, Fumin, Cousineau, Christopher, Xing, Gang, Raha, Nancy, Clemens, Sara, Mofikoya, Melissa, Kim, Moo-Young, Zhang, Jenny Yanhua
Format Journal Article
LanguageEnglish
Published England Newlands Press 01.03.2019
Subjects
Adenosine
Biomarkers
Chromatography
Chromatography, Liquid - methods
Double-Blind Method
Fructose
Fructose - chemistry
Gas flow
Humans
Hydrophobic and Hydrophilic Interactions
Insulin resistance
Ions
Laboratories
Liquid chromatography
Liver diseases
Mass spectrometry
Metabolic disorders
Metabolic syndrome
Metabolites
Mortality
Reproducibility of Results
Scientific imaging
Sorbitol
Sorbitol - chemistry
Tandem Mass Spectrometry - methods
United States > US
fructose
NASH
biomarker
HILIC
UPLC–MS/MS
sorbitol
fit-for-purpose validation
human plasma
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Summary:Fructose and sorbitol are utilized as biomarkers for nonalcoholic steatohepatitis. Measurement of fructose and sorbitol levels helps understanding disease progression, drug response and underlying mechanism. Stable isotope-labeled fructose and sorbitol were used as surrogate standards and internal standards. Human plasma samples were processed and analyzed by ultra performance LC -MS/MS via chromatographic separation on a hydrophilic interaction liquid chromatography analytical column without derivatization. Assay was validated with biomarker fit-for-purpose concept. A 12-min ultra performance LC -MS/MS method was developed and validated to directly measure fructose and sorbitol in human plasma with acceptable intra- and inter-assay precision and accuracy. This sensitive, selective, and high-throughput assay with suitable dynamic ranges was successfully applied to clinical studies to provide reliable fructose and sorbitol biomarker data.
Bibliography:ObjectType-Article-1
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ISSN:1757-6180
1757-6199
DOI:10.4155/bio-2018-0286