Epidemiological, serological, and entomological aspects of visceral leishmaniasis (VL) in suspected new VL foci in Bangladesh

• Published in: Acta tropica Vol. 248; p. 107021
• Main Authors: Ghosh, Debashis, Rashid, Md Utba, Sagar, Soumik Kha, Uddin, Md Rasel, Maruf, Shomik, Ghosh, Prakash, Chowdhury, Rajashree, Rahat, Md Abu, Islam, Md Nazmul, Aktaruzzaman, M.M., Sohel, Abu Nayeem Mohammad, Banjara, Megha Raj, Kroeger, Axel, Aseffa, Abraham, Mondal, Dinesh
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2023
• Subjects: Animals; Bangladesh; Bangladesh - epidemiology; Female; Fever; Humans; India - epidemiology; Leishmaniasis, Visceral - epidemiology; Leishmaniasis, Visceral - parasitology; New foci; Phlebotomus; Psychodidae; Sandfly; Visceral leishmaniasis; VL transmission; Bangladesh; India; VL transmission; Bangladesh; New foci; Visceral leishmaniasis; Sandfly
• ISSN: 0001-706X; 1873-6254
• DOI: 10.1016/j.actatropica.2023.107021

•The ongoing activities of NKEP are focused on endemic areas in Bangladesh.•Transmission of the disease is likely to occur in non-programmatic areas.•Epidemiological, serological, and entomological investigation is necessary in non-programmatic areas.•Asymptomatic carriers and the disease vector have been found in these areas.•NKEP should continue program activities in the newly identified foci to prevent disease transmission. The study aimed to explore epidemiological, serological, and entomological aspects of visceral leishmaniasis (VL) in suspected new VL foci and assess the knowledge, attitude, and practices of the community living in the alleged new VL foci. The study investigated new visceral leishmaniasis (VL) cases reported between 2019 and 2020 in four sub-districts (Dharmapasha, Hakimpur, Islampur and Savar) where we tested 560 members using the rK39 rapid test and conducted vector collections in six neighbouring houses of the index cases to assess sandfly density and distribution, examined sandflies' infection, and determined the spatial relationship with VL infection. Furthermore, we highlighted the importance of early detection, and community awareness in controlling the spread of the disease. The study screened 1078 people from 231 households in the four sub-districts for fever, history of visceral leishmaniasis (VL), and PKDL-like skin lesions. Among sub-districts, positivity rate for rK39 rapid test was highest (3.5 %) in Savar. Sandflies were present across all areas except in Dharmapasha, but all 21 collected female P. argentipes sandflies were negative for Leishmania parasite DNA. We found one person from Islampur with a history of VL, and one from Islampur and another one from Savar had PKDL. After the awareness intervention, more people became familiar with VL infection (91.2 %), and their knowledge concerning sandflies being the vector of the disease and the risk of having VL increased significantly (30.1 %). The study found no active case in the suspected new foci, but some asymptomatic individuals were present. As sandfly vectors exist in these areas, the National Kala-azar Elimination Programme (NKEP) should consider these areas as kala-azar endemic and initiate control activities as per national guidelines.