Micro Array-Assisted Analysis of Anti-Schistosome Glycan Antibodies Elicited by Protective Vaccination With Irradiated Cercariae

• Published in: The Journal of infectious diseases Vol. 219; no. 10; pp. 1671 - 1680
• Main Authors: Yang, Y Y Michelle, Wilson, R Alan, Thomas, Steffan R L, Kariuki, Thomas M, van Diepen, Angela, Hokke, Cornelis H
• Format: Journal Article
• Language: English
• Published: United States Oxford University Press 19.04.2019
• Subjects: Animals; Antibodies, Helminth - immunology; Antigens; Antigens, Helminth - immunology; Cercaria - immunology; Cercaria - radiation effects; Immunization; Immunoglobulin G; Immunoglobulin G - immunology; Immunoglobulin M; Immunoglobulin M - immunology; Microarray Analysis - methods; Papio anubis - immunology; Papio anubis - parasitology; Polysaccharides; Polysaccharides - immunology; Polysaccharides - metabolism; Schistosoma mansoni - immunology; Schistosoma mansoni - radiation effects; Schistosomiasis mansoni - immunology; Schistosomiasis mansoni - prevention & control; Schistosomiasis mansoni - veterinary; Vaccination; glycan; baboon vaccination; glycan-microarray; irradiated cercariae; antibodies; schistosomula; schistosomiasis; anti-glycan response; vaccination
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1093/infdis/jiy714

Baboons vaccinated with radiation-attenuated cercariae develop high levels of protection against schistosome infection, correlating to high antibody titres towards schistosome antigens with unknown molecular identity. Using a microarray consisting of glycans isolated from different life-stages of schistosomes, we studied the anti-glycan immunoglobulin (Ig) G and IgM responses in vaccinated and challenged baboons over a time course of 25 weeks. Anti-glycan IgM responses developed early after vaccination, but did not rise in response to later vaccinations. In contrast, anti-glycan IgG developed more slowly, but was boosted by all five subsequent vaccinations. High IgM and IgG levels against O-glycans and glycosphingolipid glycans of cercariae were observed. At the time of challenge, while most antibody levels decreased in the absence of vaccination, IgG towards a subset of glycans containing multiple-fucosylated motifs remained high until 6 weeks post-challenge during challenge parasite elimination, suggesting a possible role of this IgG in protection.