Meta-transcriptome-based molecular characterization of a novel fijivirus detected in a Sogatella furcifera population from Myanmar

• Published in: Journal of Asia-Pacific entomology Vol. 27; no. 3; pp. 102302 - 5
• Main Authors: Wu, Kuo, Chen, Yongdui, Zhang, Lizhen, Li, Qiannan, Yin, Zar Chi Htwe, Yin, Yanqiong, Dong, Jiahong
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.2024; 한국응용곤충학회
• Subjects: amino acid sequences; entomology; family; genome; genus; Myanmar; Nilaparvata lugens reovirus; phylogeny; RNA; Sogatella furcifera; species; viruses; 농학; Myanmar
• ISSN: 1226-8615; 1876-7790
• DOI: 10.1016/j.aspen.2024.102302

Phylogeny of SFRV with other selected reoviruses. Unrooted phylogenetic trees based on RdRp amino acid sequences of selected reoviruses were constructed in MEGA X using the neighbour-joining method and 1000 bootstrap replicates. MRDV, maize rough dwarf virus; RBSDV, rice black streaked dwarf virus; SRBSDV, southern rice black streaked dwarf virus; MDRCV, Mal de Río Cuarto virus, FDV, Fiji disease virus; NLRV,Nilaparvata lugens reovirus; DcRV, Diaphorina citri reovirus; PARV, Psammotettix alienus reovirus; RRSV, rice ragged stunt virus; RGDV, rice gall dwarf virus; RDV, rice dwarf virus; HoVRV, Homalodisca vitripennis reovirus. [Display omitted] •A novel fijivirus was detected in a Sogatella furcifera population collected.•SFRV was consisted of a near-complete genome of 10 ds RNA segments.•SFRV is the first insect-specific reovirus infecting S. furcifera.•It is a non-pathogenic virus that does not multiply nor cause disease in rice plants. A novel double- stranded RNA (dsRNA) virus, tentatively named “Sogatella furcifera reovirus” (SFRV), was detected using meta-transcriptome method in a Sogatella furcifera collected in Yangon, Myanmar. The SFRV genome comprises 10 dsRNA segments encoding 11 putative proteins. The 11 ORFs and their encoded proteins has 49.1%-71.0% nucleotide and 30.5%-76.3% amino acid sequence identities with those of reference fijiviruses. Phylogenetic analysis revealed that SFRV clustered together with Nilaparvata lugens reovirus based on the RdRp. Fijivirus-specific motifs were identified in the SFRV genome. Thus, according to the described species demarcation criteria, SFRV belongs to a new virus in the genus Fijivirus, family Reoviridae. This is first report of an insect-specific fijivirus in S. furcifera.