Pheromone biosynthesis activating neuropeptide induces attractant production in female adults of Bemisia tabaci (MED)

• Published in: Journal of Asia-Pacific entomology Vol. 28; no. 1; p. 102361
• Main Authors: Cha, Wook Hyun, Jeong, In Hong, Lee, Dae-Weon
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.03.2025; 한국응용곤충학회
• Subjects: Attractants; Bemisia tabaci; biosynthesis; entomology; females; GC–MS; genes; hexane; neuropeptides; pepper; pests; Pheromone biosynthesis activating neuropeptide; pheromones; Tomato chlorosis virus; Tomato yellow leaf curl virus; transcriptome; viruses; 농학; Attractants; Pheromone biosynthesis activating neuropeptide; GC–MS; Bemisia tabaci
• ISSN: 1226-8615; 1876-7790
• DOI: 10.1016/j.aspen.2024.102361

[Display omitted] •This study aims to identify attractants from Bemisia tabaci (MED).•Pheromone biosynthesis activating neuropeptide was cloned from female adults of B. tabaci.•PBAN-inducing compounds can be used as attractants for the control of B. tabaci. Bemisia tabaci is a polyphagous pest that is distributed worldwide and transmits various viruses, including tomato yellow leaf curl virus, tomato chlorosis virus, pepper whitefly-borne vein yellows virus and so on. We analyzed the transcriptome of B. tabaci and identified pheromone biosynthesis activating neuropeptide (PBAN) gene from female adults. Like other hemipterans, Bta-PBAN harbored four neuropeptides (DH-like peptide, PBAN, β-SGNP, and γ-SGNP). The artificial Bta-PBAN was injected into female adults, and compounds were extracted using hexane. Subsequently, GC–MS analysis was performed to identify putative attractants from the extracted compounds. Totally 22 compounds were up-regulated after artificial Bta-PBAN injection. Among them, 2-ethylhexanoic acid (2-EHA) and phytol were selected as putative attractants of B. tabaci adults. 2-EHA and phytol showed the highest attraction rate at 10% and 30% concentration, respectively. However, their attractancy did not depend on the concentration. This research is the first report on identifying attractants derived from B. tabaci. These results suggest that 2-EHA and phytol can be used as attractants for B. tabaci.