Determination of serum aflatoxin B1-lysine to evaluate the efficacy of an aflatoxin-adsorbing feed additive in pigs fed an aflatoxin B1-contaminated diet

• Published in: Mycotoxin research Vol. 33; no. 2; pp. 93 - 102
• Main Authors: Di Gregorio, Mayra Carraro, Jager, Alessandra Vincenzi, Souto, Pollyana Cristina Maggio Castro, Costa, Aline Alves, Rottinghaus, George Edwin, Passarelli, Danielle, Budiño, Fabio Enrique Lemos, Corassin, Carlos Humberto, Oliveira, Carlos Augusto Fernandes
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.05.2017
• Subjects: Biomedical and Life Sciences; Chemistry/Food Science; Life Sciences; Medical Microbiology; Medicine/Public Health; Microbiology; Original Article; LC-MS/MS; Adsorbent; Swine; AFB; lysine adduct
• ISSN: 0178-7888; 1867-1632
• DOI: 10.1007/s12550-016-0267-5

In this study, serum aflatoxin B 1 (AFB 1 )-lysine was determined in order to evaluate the in vivo efficacy of a hydrated sodium calcium aluminosilicate (HSCAS) in pigs fed AFB 1 . Twenty-four 49-day-old crossbred barrows were maintained in individual cages and allowed ad libitum access to feed and water. A completely randomized design was used with six animals assigned to each of four dietary treatments for 21 days as follows: (A) basal diet (BD), (B) BD supplemented with 0.5 % HSCAS, (C) BD supplemented with 1.1 mg/kg AFB 1 , and (D) BD supplemented with 0.5 % HSCAS and 1.1 mg/kg AFB 1 . HSCAS was able to alleviate the toxic effects of AFB 1 on pigs and reduce ( P  < 0.05) the levels of serum AFB 1 -lysine. Cumulative reductions of adduct yield values, calculated through the equation [(pg AFB 1 -lysine/mg albumin) / (μg AFB 1 /kg body weight)], were 53.0, 62.8, and 72.1 after 7, 14, and 21 days of oral exposure, respectively. AFB 1 -lysine has potential as an AFB 1 -specific biomarker for diagnostic purposes and for evaluating the efficacy of chemoprotective interventions in pigs.