Interspecies relations between Bacillus thuringiensis strains studied by AP-PCR and sequence analysis of ribosomal operon regions
The interspecies relationships between Bacillus thuringiensis strains producing different types of δ-endotoxins were studied using a range of molecular-biological methods. Analysis of the 16S rRNA nucleotide sequence, the 16S to 23S rRNA intergenic spacer sequence, and the 5′-terminal region of 23S...
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Published in | Microbiology (New York) Vol. 78; no. 6; pp. 703 - 710 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
SP MAIK Nauka/Interperiodica
01.12.2009
|
Subjects | |
Online Access | Get full text |
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Summary: | The interspecies relationships between
Bacillus thuringiensis
strains producing different types of δ-endotoxins were studied using a range of molecular-biological methods. Analysis of the 16S rRNA nucleotide sequence, the 16S to 23S rRNA intergenic spacer sequence, and the 5′-terminal region of 23S rRNA allowed the studied strains to be subdivided into three groups based on the pattern of nucleotide substitutions. In terms of the pattern of substitutions, the strains of the first group are similar to the
B. thuringiensis
type strain ATCC 10792
T
, the strains of the second group are practically identical to
B. anthracis
and the
B. cereus
type strain ATCC 14579
T
, whereas the third group combines strains of
B. thuringiensis
subsp.
morrisoni
with the
cry2
gene and strains of
B. thuringiensis
subsp.
tenebrionis
with the
cry3
gene. PCR fingerprinting with the use of six different primer systems ((GTG)
5
, REP, ERIC, and DIR) confirmed the presence of three statistically relevant groups, whose structure correlated with that suggested by the analysis of ribosomal operon regions. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0026-2617 1608-3237 |
DOI: | 10.1134/S002626170906006X |