Pioglitazone Eases Testicular Torsion/Detorsion-Induced Ischemia- Reperfusion Injury in Rats

• Published in: Journal of urological surgery Vol. 10; no. 2; pp. 167 - 172
• Main Authors: Senturk, Irfan Yildirim, Deger, Muslim Dogan, Aydin, Muhammed Ali, Madendere, Serdar, Kaya, Oktay, Tastekin, Ebru, Aktoz, Tevfik
• Format: Journal Article
• Language: English
• Published: Galenos Yayinevi Tic. Ltd 01.06.2023; Galenos Yayinevi
• Subjects: Complications and side effects; Drug therapy; ischemia; reperfusion; Reperfusion injury; Risk factors; Spermatic cord torsion; testicular torsion; Turkey
• ISSN: 2148-9580; 2148-9580
• DOI: 10.4274/jus.galenos.2022.2022.0047

What's known on the subject? and What does the study add? Ischemia-reperfusion-induced changes in testicular histological parameters have been shown to be improved by pioglitazone 6 mg/kg therapy. Pioglitazone can be used in emergencies such as torsion and other chronic diseases to improve testicular functions. Objective: This study demonstrated the protective effects of pioglitazone (Pio) on testicular torsion/detorsion-induced ischemia-reperfusion (I/R) injury. Materials and Methods: A total of 48 male rats were randomly divided into six experimental groups of eight rats each; Control group, I/R group, Pio 3 mg/kg group, I/R treated with Pio 3 mg/kg group, Pio 6 mg/kg group, and I/R treated with Pio 6 mg/kg group. Testicular torsion was induced by twisting the left testis 720 [degrees]C in a clockwise direction (I/R groups). Both ischemia and reperfusion periods were 4 h. Single-dose 3 mg/kg Pio or 6 mg/kg Pio was administered orally two hours before reperfusion (Pio groups). Left orchiectomy was performed at the end of the protocol. Results: In the I/R within-group analysis for mean seminiferous tubule diameter and epithelial lengths, a statistically significant difference was found only in the Pio 6 group (p=0.005; p=0.005). But Pio treatment failed to improve the levels of malondialdehyde and glutathione. Also, it did not cause any change in the non-I/R groups. Conclusion: Considering the findings of Pio, it may be used in emergencies such as torsion and other chronic diseases. Keywords: Testicular torsion, ischemia, reperfusion