Not the usual suspect: Polymeric IgA paraprotein causes false positive results in kinetic interaction of microparticles in solution (KIMS) immunoassays

Immunoassays are commonly used by the clinical laboratory, but paraproteins can occasionally produce erroneous results. In this study, we investigated the cause of apparent false positive results for multiple Kinetic Interaction of Microparticles in Solution (KIMS) immunoassays. Patient controls and...

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Bibliographic Details
Published inClinical biochemistry Vol. 92; pp. 82 - 85
Main Authors Kemble, David J., Lamothe, Simon, Uhl, Lynne
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.06.2021
Subjects
Falsely elevated
IgA paraprotein
Immunoassay interference
Kinetic Interaction of Microparticles in Solution (KIMS) immunoassays
Multiple myeloma
Therapeutic drugs
Kinetic Interaction of Microparticles in Solution (KIMS) immunoassays
Therapeutic drugs
Falsely elevated
Immunoassay interference
Multiple myeloma
IgA paraprotein
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Summary:Immunoassays are commonly used by the clinical laboratory, but paraproteins can occasionally produce erroneous results. In this study, we investigated the cause of apparent false positive results for multiple Kinetic Interaction of Microparticles in Solution (KIMS) immunoassays. Patient controls and samples containing the interference were analyzed using automated chemistry platforms, gel electrophoresis, immunofixation, affinity chromatography, and size exclusion chromatography. Our results show that IgA paraprotein caused false positive results for the KIMS measurement of three therapeutic drugs. To our knowledge, this is the first report of IgA paraprotein-causing immunoassay interference. The clinical implications of this interference are discussed.
Bibliography:ObjectType-Case Study-2
SourceType-Scholarly Journals-1
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ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2021.03.001