Species-specific molecular differentiation of embryonic inner cell mass and trophectoderm: A systematic review

• Published in: Animal reproduction science Vol. 252; p. 107229
• Main Authors: Marsico, Thamiris Vieira, Valente, Roniele Santana, Annes, Kelly, Oliveira, Andressa Minozzo, Silva, Mara Viana, Sudano, Mateus José
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2023
• Subjects: Animals; Blastocyst; Cattle; Cell Differentiation - genetics; Cell fate; Cell Lineage; Cellular differentiation; Early embryo development; Embryo, Mammalian; Embryonic Development - genetics; Female; Gene Expression Regulation, Developmental; Horses - genetics; Humans; Mammals; Mice; Molecular characterization; Pregnancy; PRISMA; Swine; Early embryo development; PRISMA; Cell fate; Molecular characterization; Cellular differentiation
• ISSN: 0378-4320; 1873-2232
• DOI: 10.1016/j.anireprosci.2023.107229

A wide-ranging review study regarding the molecular characterization of the first cell lineages of the developmental embryo is lacking, especially for the primary events during earliest differentiation which leads to the determination of cellular fate. Here, a systematic review and meta-analysis were conducted according to PRISMA guidelines. MEDLINE-PubMed was searched based on an established search strategy through April 2021. Thirty-six studies fulfilling the inclusion criteria were subjected to qualitative and quantitative analysis. Among the studies, 50 % (18/36) used mice as an animal model, 22.2 % (8/36) pigs, 16.7 % (6/36) cattle, 5.5 % (2/36) humans, and 2.8 % (1/36) goats as well as 2.8 % (1/36) equine. Our results demonstrated that each of the first cell lineages of embryos requires a certain pattern of expression to establish the cellular determination of fate. Moreover, these patterns are shared by many species, particularly for those molecules that have already been identified in the literature as biomarkers. In conclusion, the present study integrated carefully chosen studies regarding embryonic development and first cellular decisions in mammalian species and summarized the information about the differential characterization of the first cell lineages and their possible relationship with specific gene expression.