Antimicrobial activity of acrylic intraocular lenses soaked in fourth generation fluoroquinolones

• Published in: Journal of ocular pharmacology and therapeutics Vol. 24; no. 5; p. 495
• Main Authors: Smith, Edward F, Elbash, Abdel Rahman, Schrier, Amilia, Berg, Peter D, Eid, Issa
• Format: Journal Article
• Language: English
• Published: United States 01.10.2008
• Subjects: Acrylic Resins; Administration, Topical; Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - pharmacokinetics; Aqueous Humor - metabolism; Aza Compounds - administration & dosage; Aza Compounds - pharmacokinetics; Drug Delivery Systems; Fluoroquinolones - administration & dosage; Fluoroquinolones - pharmacokinetics; In Vitro Techniques; Lenses, Intraocular; Quinolines - administration & dosage; Quinolines - pharmacokinetics; Staphylococcus aureus - drug effects; Staphylococcus aureus - isolation & purification
• ISSN: 1557-7732
• DOI: 10.1089/jop.2008.0007

The aim of this study was to determine whether a commercially available brand of intraocular lenses (IOLs) soaked in moxifloxacin or gatifloxacin for 1 or 60 min have antimicrobial properties. The IOLs (N = 8-10/group) were soaked in saline, physiologic-strength moxifloxacin (1.8 microg/mL), or gatifloxacin (0.48 microg/mL) for 60 min or commercial-strength moxifloxacin (5 mg/mL) or gatifloxacin (3 mg/mL) for 1 or 60 min. Presoaked IOLs and gatifloxacin antibiotic disks were plated on agar with quality-controlled ATCC 25923 Staphylococcus aureus overlay. Bacterial kill zones were measured after 24 h. IOLs soaked in physiologic-strength gatifloxacin or saline for 60 min produced no measurable bacterial kill zone, and the mean bacterial kill zone for IOLs soaked in physiologic-strength moxifloxacin was significantly greater (P = 0.011, 0 vs. 3.88 +/- 3.18 mm, respectively). Soaking the IOLs in commercial-strength moxifloxacin or gatifloxacin for 1 or 60 min produced significantly larger bacterial kill zones (P < 0.0001, mean: > or =33 mm for all groups). Soaking for 1 min produced a significantly larger mean bacterial kill zone by moxifloxacinthan gatifloxacin-treated IOLs (P = 0.002, 38.80 +/- 3.74 mm, 34.30 +/- 1.34 mm, respectively). The mean bacterial kill zone was significantly larger for IOLs soaked in commercial-strength moxifloxacin for 1 versus 60 min (P = 0.002, 38.80 +/- 3.74 mm, 33.56 +/- 1.42 mm, respectively). There was no significant difference in the mean bacterial kill zone between IOLs soaked for 1 or 60 min in commercial-strength gatifloxacin (34.30 +/- 1.34 mm, 33.67 +/- 0.50 mm, respectively).