Establishment of import tolerance for the insecticide thiacloprid in strawberry

• Published in: Biomedical chromatography Vol. 35; no. 5; pp. e5057 - n/a
• Main Authors: Rahman, Md. Musfiqur, Oh, Young Goun, Lee, Dong‐Gi, Moon, Joon‐Kwan, Shim, Jae‐Han, Cho, Il Kyu
• Format: Journal Article
• Language: English
• Published: England 01.05.2021
• Subjects: export promotion; OECD MRL calculator; residual pattern; Thiacloprid; UPLC–MS/MS; OECD MRL calculator; export promotion; UPLC-MS/MS; residual pattern; Thiacloprid
• ISSN: 0269-3879; 1099-0801
• DOI: 10.1002/bmc.5057

To promote exports, import tolerance (IT) of thiacloprid in strawberry was proposed using the Organization for Economic Cooperation and Development (OECD) maximum residue limit (MRL) calculator after conducting three different field trials. The pre‐harvest interval of residual pattern and degradation dynamics of thiacloprid in strawberry were determined using ultra‐performance liquid chromatography–tandem mass spectrometry. Samples were extracted with acetonitrile and a mixture of salts and dilution was performed for purification. A six‐point matrix‐matched calibration curve was constructed which provided excellent linearity with coefficient of determination (R2) of 0.9998 or more. Detection and quantification limits were 0.003 and 0.01 mg/kg, respectively. The method was validated in quintuplicate at three different concentrations, which resulted in acceptable recovery ranging from 80.86% to 101.71% with relative standard deviation of 6.50 or less among the three field sites. The developed method was applied to the field‐treated sample harvested at different intervals. In the pre‐harvest interval trial, the amount of thiacloprid residues ranged from 0.24 to 0.70 mg/kg in field site 1 (Nonsan), 0.16 to 0.50 mg/kg in field site 2 (Sunchang), and 0.36 to 0.50 mg/kg in field site 3 (Sacheon). By contrast, in the degradation trial, the observed residues were 0.03–0.81 mg/kg in field site 1 and 0.02–0.48 mg/kg in field site 2. Consequently, the IT of thiacloprid in strawberry using the OECD MRL calculator was proposed as 2 mg/kg, which is exactly the same as the MRL established by the Republic of Korea. In conclusion, the residue study proposes 2.0 mg/kg as the MRL of thiacloprid in strawberries.