Human lymphocyte differentiation antigens HB-10 and HB-11. I. Ontogeny of antigen expression

• Published in: The Journal of immunology (1950) Vol. 134; no. 5; pp. 2983 - 2988
• Main Authors: Tedder, TF, Clement, LT, Cooper, MD
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 01.05.1985; American Association of Immunologists
• Subjects: Adult; Analysis of the immune response. Humoral and cellular immunity; Antibodies, Monoclonal - biosynthesis; Antigen-Antibody Reactions; Antigens, Differentiation, T-Lymphocyte; Antigens, Neoplasm - analysis; Antigens, Neoplasm - immunology; Antigens, Surface - analysis; Antigens, Surface - immunology; B-Lymphocytes - cytology; B-Lymphocytes - immunology; Biological and medical sciences; Cell Differentiation; Cell Line; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Hematopoietic Stem Cells - immunology; Humans; Hybridomas - metabolism; Immunobiology; Infant, Newborn; Leukemia - immunology; Lymphoma - immunology; Organs and cells involved in the immune response; T-Lymphocytes, Helper-Inducer - classification; T-Lymphocytes, Helper-Inducer - cytology; T-Lymphocytes, Helper-Inducer - immunology; Antigen; Cell function; T-Lymphocyte; Development; Biological marker; Helper cell; Monoclonal antibody; Cell differentiation; Cell subpopulation
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.134.5.2983

T, B, and NK cells appear to represent separate lymphocyte lineages, but indirect evidence suggests that they may be related via a common lymphoid precursor cell. We have produced two monoclonal antibodies, HB-10 (IgM) and HB-11 (IgG1), by fusing spleen cells from mice immunized with the human B cell line SB, and have shown that both antibodies react with lymphocyte-specific cell surface antigens present on T, B, and NK cells, but not on other types of blood cells. The antibodies were reactive with most cell lines and malignancies of B cell origin and with some of T and NK cell lineage. Although the populations of cells expressing these two antigens were virtually identical, the HB-10 and HB-11 antibodies identified separate protease-sensitive determinants on the cell surface. The HB-11 antigenic determinant was also sensitive to neuraminidase and periodate treatments, but the HB-10 determinant was not. Antigen expression by lymphocytes from fetal, newborn, and adult tissues was examined. Within the B cell lineage, these antigens were expressed by most pre-B cells in bone marrow (88% +/- 5) and almost all B cells, but were not expressed by mature plasma cells. Virtually all of the granular lymphocytes in blood marked by the Leu-7 and Leu-11 (anti-Fc receptor) antibodies were HB-10+ and 11+. Among T lineage cells, the HB-10 and 11 antigens were expressed by a subset of relatively mature T3+ thymocytes and by greater than 90% of the T cells in newborn blood. In adults, however, only 65% of blood T cells and 24 to 30% of splenic or tonsillar T cells expressed the HB-10 and HB-11 antigens. The postnatal emergence of T cells which, like plasma cells, do not express these antigens suggests that post-thymic T lymphocyte maturation occurs and may be an activation-dependent process.