LC/QTOF profile and preliminary stability studies of an enriched flavonoid fraction of Cecropia pachystachya Trécul leaves with potential antidepressant‐like activity

• Published in: Biomedical chromatography Vol. 31; no. 11
• Main Authors: Ortmann, Caroline Flach, Abelaira, Helena Mendes, Réus, Gislaine Zilli, Ignácio, Zuleide Maria, Chaves, Vitor Clasen, Santos, Talitha Caldas, Carvalho, Pâmela, Carlessi, Anelise Scussel, Bruchchen, Livia, Danielski, Lucineia G., Cardoso, Simone Gonçalves, Campos, Angela Machado, Petronilho, Fabricia, Rebelo, Joyce, Santos Morais, Meline Oliveira, Vuolo, Francieli, Dal‐Pizzol, Felipe, Streck, Emilio Luiz, Quevedo, João, Reginatto, Flávio Henrique
• Format: Journal Article
• Language: English
• Published: England 01.11.2017
• Subjects: Animals; Antidepressive Agents - analysis; Antidepressive Agents - chemistry; Antidepressive Agents - pharmacology; Behavior, Animal - drug effects; Brain Chemistry - drug effects; Cecropia pachystachya; Cecropia Plant - chemistry; Chromatography, Liquid - methods; Cytokines - analysis; C‐glycosyl flavonoids; Drug Stability; Flavonoids - analysis; Flavonoids - chemistry; Flavonoids - pharmacology; isoorientin; major depressive disorder; Male; oxidative stress; Oxidative Stress - drug effects; Plant Extracts - chemistry; Plant Extracts - pharmacology; Rats; Rats, Wistar; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; isoorientin; C-glycosyl flavonoids; Cecropia pachystachya; major depressive disorder; oxidative stress
• ISSN: 0269-3879; 1099-0801
• DOI: 10.1002/bmc.3982

There is increasing interest in natural antioxidants that are candidates for the prevention of brain damage occurring in major depressive disorders. Cecropia pachystachya is a tropical tree species of Central and South America and a rich source of polyphenols, particularly flavonoids. The aim of this study was to characterize the flavonoid profile of an enriched flavonoid fraction of C. pachystachya (EFF‐Cp) and evaluate the antidepressant‐like effects of its acute administration in behavior, cytokine levels, oxidative stress and energy metabolism parameters. The EFF‐Cp chemical characterization was performed by HPLC/DAD and LC/QTOF. The antidepressant‐like effects were performed by the forced swimming test, splash test and open field test. EFF‐Cp revealed 15 flavonoids, including seven new glycosyl flavonoids for C. pachystachya. Quantitatively, EFF‐Cp showed isoorientin (43.46 mg/g), orientin (23.42 mg/g) and isovitexin (17.45 mg/g) as major C‐glycosyl flavonoids. In addition, EFF‐Cp at doses 50 and 100 mg/kg reduced the immobility time in the forced swimming test, without changing the locomotor activity and grooming time. In addition, EFF‐Cp was able to prevent the oxidative damage in some brain areas. In conclusion, the results of this study suggest that EFF‐Cp exerts antidepressant‐like effects with its antioxidant properties.