Simultaneous pharmacokinetic assessment of phytopharmaceuticals in fenugreek extract using LC–MS/MS in Sprague–Dawley rats

Fenugreek seeds are used in numerous marketed herbal formulations with therapeutic benefits. Some of its bioactive components such as 4‐hydroxyisoleucine, trigonelline, raffinose, and pinitol are reported to possess potential therapeutic activities, such as antibacterial, antidiabetic, stomach stimu...

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Published inBiomedical chromatography Vol. 37; no. 5; pp. e5600 - n/a
Main Authors Agrawal, Sristi, Bisen, Amol Chhatrapati, Biswas, Arpon, Sanap, Sachin Nashik, Verma, Sarvesh Kumar, Kumar, Mukesh, Jaiswal, Shubham, Kumar, Ashok, Narender, Tadigoppula, Bhatta, Rabi Sankar
Format Journal Article
LanguageEnglish
Published England 01.05.2023
Subjects
4‐hydroxyisoleucine
Animals
Chromatography, High Pressure Liquid - methods
Chromatography, Liquid - methods
Drugs, Chinese Herbal - pharmacokinetics
Female
LC–MS/MS
pinitol
Plant Extracts - pharmacokinetics
polycystic ovary syndrome
raffinose
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Tandem Mass Spectrometry - methods
Trigonella foenum‐graecum
trigonelline
LC-MS/MS
Trigonella foenum-graecum
4-hydroxyisoleucine
trigonelline
pinitol
raffinose
polycystic ovary syndrome
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Summary:Fenugreek seeds are used in numerous marketed herbal formulations with therapeutic benefits. Some of its bioactive components such as 4‐hydroxyisoleucine, trigonelline, raffinose, and pinitol are reported to possess potential therapeutic activities, such as antibacterial, antidiabetic, stomach stimulant, and anti‐invasive, against hyperandrogenism and other allied diseases, including polycystic ovary syndrome. A fully validated, selective, and sensitive bioanalytical method for the simultaneous rapid quantification of the aforementioned bioactive components has been developed using hyphenated liquid chromatography electrospray tandem mass spectrometry. The analytes were separated within 5 min using gradient elution in a C18 column at a flow rate of 0.5 ml/min. Plasma protein precipitation technique was employed to isolate the analytes from the samples. Oral pharmacokinetic profile of the four bioactive components in Sprague–Dawley rats was further evaluated using noncompartmental analysis using Phoenix WinNonlin software.
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ISSN:0269-3879
1099-0801
1099-0801
DOI:10.1002/bmc.5600