Effect of Bu-Zhong-Yi-Qi-Tang on deficiency of N-glycan/ nitric oxide and islet damage induced by streptozotocin in diabetic rats

• Published in: World journal of gastroenterology : WJG Vol. 15; no. 14; pp. 1730 - 1737
• Main Authors: Liu, Xiao-Qiu, Wu, Ling, Guo, Xue-Jun
• Format: Journal Article
• Language: English
• Published: United States Pi-Wei Institute, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong Province, China%Laboratory Animal Research Center, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, Guangdong Province, China 14.04.2009; The WJG Press and Baishideng
• Subjects: alpha-Mannosidase - metabolism; Amylases - metabolism; Animals; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - metabolism; Drugs, Chinese Herbal - therapeutic use; Humans; Islets of Langerhans - cytology; Islets of Langerhans - metabolism; Islets of Langerhans - pathology; Lectins - metabolism; Nitrates - metabolism; Nitric Oxide - metabolism; Nitrites - metabolism; Original; Polysaccharides - metabolism; Rats; Rats, Sprague-Dawley; 一氧化氮; 淀粉酶升高; 甘露糖苷酶; 益气汤; 糖尿病大鼠; 胰岛损伤; 酶活性测定; 链脲佐菌素; Islet damage; Bu-Zhong-Yi-Qi-Tang; Diabetic rats; Alpha mannosidase; N-Glycan; Nitric oxide
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.15.1730

AIM： To investigate the effect of Bu-Zhong-Yi-Qi-Tang （Decoction for Reinforcing Middle Jiao and Replenishing Qi） on deficiency of N-glycan/nitric oxide （NO） and islet damage induced by injecting two medium doses of streptozotocin （STZ）. METHODS： Diabetes was induced by intraperitoneal injection of STZ at 55 mg/kg on day 1 and day 8. Islet damage was evaluated using a scoring system. Nitrite, nitrate, α-mannosidase and amylase activities were measured by colorimetry. N-glycan patterns of amylase were determined with lectin [ConA, pisum sativum agglutinin （PSA）, peanut agglutinin （PNA）, and lens culinaris agglutinin （LCA）] affinity precipitation method. RESULTS： Severe islet necrosis and mild islet atrophy were observed in diabetic rats. The number and size of islets, the activities of α-mannosidase, amylase and nitrite were decreased, while the binding of PNA and LCA to amylase was increased. All of which were improved after treatment with Bu-Zhong-Yi-Qi-Tang. Islet damage was significantly correlated with nitrite, nitrate, α-mannosidase, amylase and the binding of LCA, PNA, and PSA to amylase.CONCLUSION： STZ-induced islet damage is related to N-glycan deficiency in proteins by blocking α-mannosidase activity and No deficiency, accumulation of unfolded proteins, and endoplasmic reticulum stress and activation of cellular signals, all of which are improved after treatment with Bu-Zhong-Yi- Qi-Tang.